Quantification of residual dimethyl sulfoxide in supernatants of haematopoietic stem cells by capillary zone electrophoresis

Dimethyl sulfoxide (DMSO) is a chemical compound that is used to preserve haematopoietic stem cells during freezing at −180°C. As DMSO is largely removed by washing before reinjection of cells into a patient, accidents (notably cardiovascular) are infrequent. The lack of a method for evaluating the...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1999-05, Vol.728 (1), p.75-83
Main Authors: Houzé, Pascal, Dal Cortivo, Liliane, Anselme, Michel, Bousquet, Bernard, Gourmel, Bernard
Format: Article
Language:English
Subjects:
Culture Media - chemistry
Dimethyl sulfoxide
Dimethyl Sulfoxide - analysis
Electrophoresis, Capillary - methods
Hematopoietic Stem Cells - cytology
Humans
Reproducibility of Results
Sensitivity and Specificity
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Summary:Dimethyl sulfoxide (DMSO) is a chemical compound that is used to preserve haematopoietic stem cells during freezing at −180°C. As DMSO is largely removed by washing before reinjection of cells into a patient, accidents (notably cardiovascular) are infrequent. The lack of a method for evaluating the residual quantities of this product led us to develop a technique for assaying DMSO by capillary zone electrophoresis without extraction. This simple, rapid and precise technique was applied to the supernatant of cell pellets of thirteen patients before and after washing.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(99)00092-4