Rescue of Cyclin D1 Deficiency by Knockin Cyclin E

D-type cyclins and cyclin E represent two very distinct classes of mammalian G1 cyclins. We have generated a mouse strain in which the coding sequences of the cyclin D1 gene ( Ccnd1) have been deleted and replaced by those of human cyclin E ( CCNE). In the tissues and cells of these mice, the expres...

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Bibliographic Details
Published in:Cell 1999-06, Vol.97 (6), p.767-777
Main Authors: Geng, Yan, Whoriskey, Wendy, Park, Mary Y, Bronson, Roderick T, Medema, Rene H, Li, Tiansen, Weinberg, Robert A, Sicinski, Piotr
Format: Article
Language:English
Subjects:
Animals
Cyclin D1 - deficiency
Cyclin D1 - genetics
Cyclin D1 - metabolism
Cyclin E - genetics
Cyclin E - metabolism
Humans
Mice
Mice, Inbred C57BL
Phenotype
Receptors, Estrogen - metabolism
Retina - metabolism
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Summary:D-type cyclins and cyclin E represent two very distinct classes of mammalian G1 cyclins. We have generated a mouse strain in which the coding sequences of the cyclin D1 gene ( Ccnd1) have been deleted and replaced by those of human cyclin E ( CCNE). In the tissues and cells of these mice, the expression pattern of human cyclin E faithfully reproduces that normally associated with mouse cyclin D1. The replacement of cyclin D1 with cyclin E rescues all phenotypic manifestations of cyclin D1 deficiency and restores normal development in cyclin D1–dependent tissues. Thus, cyclin E can functionally replace cyclin D1. Our analyses suggest that cyclin E is the major downstream target of cyclin D1.
ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(00)80788-6