Effects of Intragenic Variability at 3 Polymorphic Sites of the Apolipoprotein B Gene on Serum Lipids and Lipoproteins in a Multiethnic Asian Population

• Published in: Human biology 1999-06, Vol.71 (3), p.381-397
• Main Authors: CHOONG, M.L., SETHI, S.K., KOAY, E.S.C.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Alleles; Apolipoproteins B - blood; Apolipoproteins B - genetics; Asian Continental Ancestry Group - genetics; Asians; Atherosclerosis; Base Sequence; Blood lipids; Case-Control Studies; Chi-Square Distribution; China - ethnology; Cholesterols; Cohort Studies; Coronary artery disease; DNA; Female; Gene Frequency; Genetic aspects; Genetic Linkage; Genetic polymorphism; Genetic polymorphisms; Genetic Variation; Genetics; Genetics, Population; Genotypes; Humans; Hyperlipidemia; Hyperlipidemias - epidemiology; Hyperlipidemias - genetics; India - ethnology; Linkage disequilibrium; Lipids; Lipids - blood; Lipids - genetics; Lipoproteins - blood; Lipoproteins - genetics; Malaysia - ethnology; Male; Middle Aged; Molecular Sequence Data; Multivariate Analysis; Mutation; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Proteins; Singapore - epidemiology
• ISSN: 0018-7143; 1534-6617

We determined the allelic (X+/X-, M+/M-, and E+/E-) distribution frequencies of the XbaI, MspI, and EcoRI restriction fragment length polymorphisms (RFLPs) in the apolipoprotein B gene in a control group of 374 healthy Chinese, Malays, and Indians and in a hyperlipidemic cohort of 131 Chinese patients. Covariability between the RFLPs and serum lipid, lipoprotein, and apolipoprotein concentrations was also studied. We found a lower frequency (average 0.0829) of the X+ alíele and higher frequencies of the E+ (average 0.9452) and M+ (average 0.9772) alíeles in our study population compared with frequencies reported in other populations. The 3 polymorphic sites did not contribute to significant variations in lipid levels (p > 0.1 in all cases). Also, there was no significant variation in genotype frequencies between the control subjects and the hyperlipidemic subjects. Despite their relative close proximity within the APOB gene sequence, the 3 polymorphic sites did not show any significant linkage disequilibrium. However, the presence of the X + cutting site was in linkage disequilibrium with the Del alíele of the 5' insertion-deletion polymorphism and the E — alíele was in linkage disequilibrium with the 3' VNTR located near the 3' end of the coding region of the APOB gene.