oligonucleotide-ligation assay for the differentiation between Cyclospora and Eimeria spp. polymerase chain reaction amplification products

An oligonucleotide-ligation assay (OLA) was developed and compared to a restriction fragment length polymorphism (RFLP) test for distinguishing between 294-bp polymerase chain reaction (PCR) amplification products of the 18S rRNA gene from Cyclospora and Eimeria spp. The PCR/OLA correctly distinguis...

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Bibliographic Details
Published in:Journal of food protection 1999-06, Vol.62 (6), p.682-685
Main Authors: Jinneman, K.C, Wetherington, J.H, Hill, W.E, Omiescinski, C.J, Adams, A.M, Johnson, J.M, Tenge, B.J, Dang, N.L, Wekell, M.M
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cyclospora
detection
DNA, Protozoan - genetics
Eimeria
Eimeria - classification
Eimeria - genetics
Eimeria mitis
Eimeria tenella
Eimeria tenella - classification
Eimeria tenella - genetics
Eucoccidiida - classification
Eucoccidiida - genetics
food contamination
Fundamental and applied biological sciences. Psychology
General aspects and techniques
identification
Oligonucleotide Probes
oligonucleotide-ligation assay
oligonucleotides
polymerase chain reaction
Polymerase Chain Reaction - methods
Polymorphism, Restriction Fragment Length
Protozoa
RNA, Ribosomal, 18S - genetics
rRNA 18S
Sequence Analysis, DNA
strain differences
strains
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Description
Summary:An oligonucleotide-ligation assay (OLA) was developed and compared to a restriction fragment length polymorphism (RFLP) test for distinguishing between 294-bp polymerase chain reaction (PCR) amplification products of the 18S rRNA gene from Cyclospora and Eimeria spp. The PCR/OLA correctly distinguished between three Cyclospora, three E. tenella, and one E. mitis strains and the ratio of positive to negative spectrophotometric absorbance (A(490)) values for each strain ranged from 4.086 to 15.280 (median 9.5). PCR/OLA provides a rapid, reliable, spectrophotometric alternative to PCR/RFLP.
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028x-62.6.682