Short Communication: Culture-Independent Detection of Lactic Acid Bacteria Bacteriocin Genes in Two Traditional Slovenian Raw Milk Cheeses and Their Microbial Consortia

Two Slovenian traditional raw milk cheeses, Tolminc (from cows’ milk) and Kraški (from ewes’ milk), were examined for the presence of 19 lactic acid bacteria bacteriocin genes by PCR analysis of total DNA extracts from 9 cheeses and from consortia of strains isolated from these cheeses. Eleven bacte...

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Bibliographic Details
Published in:Journal of dairy science 2008-12, Vol.91 (12), p.4535-4541
Main Authors: Trmčić, A., Obermajer, T., Rogelj, I., Bogovič Matijašić, B.
Format: Article
Language:English
Subjects:
Animal productions
Animals
bacteriocin gene
Bacteriocins - genetics
Biological and medical sciences
Cattle
Cheese - microbiology
Colony Count, Microbial
DNA - analysis
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Gram-Positive Bacteria - genetics
Methods of analysis, processing and quality control, regulation, standards
Milk
polymerase chain reaction
Sheep
Slovenia
Slovenian raw milk cheese
Terrestrial animal productions
Vertebrates
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Summary:Two Slovenian traditional raw milk cheeses, Tolminc (from cows’ milk) and Kraški (from ewes’ milk), were examined for the presence of 19 lactic acid bacteria bacteriocin genes by PCR analysis of total DNA extracts from 9 cheeses and from consortia of strains isolated from these cheeses. Eleven bacteriocin genes were detected in at least one cheese or consortium, or from both. Different cheeses or consortia contained 3 to 9 bacteriocin determinants. Plantaricin A gene determinants were found in all cheese and consortia DNA extracts. Genes for enterocins A, B, P, L50A, and L50B, and the bacteriocin cytolysin were commonly detected, as were genes for nisin. These results indicate that bacteriocinogenic strains of Lactobacillus, Enterococcus, and Lactococcus genera with protective potential are common members of indigenous microbiota of raw milk cheeses, which can be a good source of new protective strains.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.2008-1396