Antioxidant activity applying an improved ABTS radical cation decolorization assay

A method for the screening of antioxidant activity is reported as a decolorization assay applicable to both lipophilic and hydrophilic antioxidants, including flavonoids, hydroxycinnamates, carotenoids, and plasma antioxidants. The pre-formed radical monocation of 2,2′-azinobis-(3-ethylbenzothiazoli...

Full description

Saved in:
Bibliographic Details
Published in:Free radical biology & medicine 1999-05, Vol.26 (9), p.1231-1237
Main Authors: Re, Roberta, Pellegrini, Nicoletta, Proteggente, Anna, Pannala, Ananth, Yang, Min, Rice-Evans, Catherine
Format: Article
Language:English
Subjects:
ABTS radical cation
Antioxidant activity
Antioxidants - pharmacology
Benzothiazoles
Cations
Color
Drug Evaluation, Preclinical - methods
Ethanol
Evaluation Studies as Topic
Flavonoid
Free radical
Free Radicals
Hydroxycinnamate
In Vitro Techniques
Indicators and Reagents
Oxidation
Polyphenol
Sulfonic Acids
TEAC
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method for the screening of antioxidant activity is reported as a decolorization assay applicable to both lipophilic and hydrophilic antioxidants, including flavonoids, hydroxycinnamates, carotenoids, and plasma antioxidants. The pre-formed radical monocation of 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS •+) is generated by oxidation of ABTS with potassium persulfate and is reduced in the presence of such hydrogen-donating antioxidants. The influences of both the concentration of antioxidant and duration of reaction on the inhibition of the radical cation absorption are taken into account when determining the antioxidant activity. This assay clearly improves the original TEAC assay (the ferryl myoglobin/ABTS assay) for the determination of antioxidant activity in a number of ways. First, the chemistry involves the direct generation of the ABTS radical monocation with no involvement of an intermediary radical. Second, it is a decolorization assay; thus the radical cation is pre-formed prior to addition of antioxidant test systems, rather than the generation of the radical taking place continually in the presence of the antioxidant. Hence the results obtained with the improved system may not always be directly comparable with those obtained using the original TEAC assay. Third, it is applicable to both aqueous and lipophilic systems.
ISSN:0891-5849
1873-4596
DOI:10.1016/S0891-5849(98)00315-3