Discriminating acute from chronic human schistosomiasis mansoni

Specific immunoglobulin (IgA, IgG and IgM) responses to different antigen targets (soluble eggs antigen – SEA, soluble worm adult protein – SWAP and keyhole limpet hole – KLH) were measured by enzyme linked immunosorbent assay (ELISA) in patients with acute and chronic schistosomiasis, as well as pa...

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Bibliographic Details
Published in:Acta tropica 2008-11, Vol.108 (2), p.229-233
Main Authors: Beck, Lílian, Van-Lüme, Daniele S.M., Souza, Joelma R., Domingues, Ana L.C., Favre, Tereza, Abath, Frederico G.C., Montenegro, Silvia M.L.
Format: Article
Language:English
Subjects:
Acute schistosomiasis
Adolescent
Adult
Aged
Animals
Antibodies, Helminth - blood
Antigens, Helminth
Area Under Curve
Ascaris
Child
Child, Preschool
Chronic schistosomiasis
Diagnosis, Differential
Differential diagnosis
Enzyme-Linked Immunosorbent Assay - methods
Female
Freshwater
Humans
Immunoglobulin A - blood
Immunoglobulin G - blood
Immunoglobulin M - blood
Male
Middle Aged
Receiver operating characteristic
ROC Curve
Schistosoma
Schistosoma mansoni
Schistosomiasis mansoni - diagnosis
Trichuris trichiura
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Summary:Specific immunoglobulin (IgA, IgG and IgM) responses to different antigen targets (soluble eggs antigen – SEA, soluble worm adult protein – SWAP and keyhole limpet hole – KLH) were measured by enzyme linked immunosorbent assay (ELISA) in patients with acute and chronic schistosomiasis, as well as patients without schistosomiasis. SEA IgA and KLH IgM presented high discriminatory powers to distinguish acute from chronic schistosomiasis, with calculated areas under the curve (AUCs) of 0.88 and 0.82, respectively, obtained from receiver operating characteristic (ROC) curve. On the other hand, these tests, particularly SEA IgA were not useful to distinguish schistosomiasis (including the acute and chronic forms) from individuals without this disease, but infected with other intestinal parasites ( Ascaris lumbricoides, Trichuris trichiura and hookworm). By contrast, SWAP IgG and SEA IgG were able to discriminate schistosomiasis patients from healthy individuals and patients infected with other parasites (AUCs of 0.96 and 0.85, respectively). Thus, it is possible to use a combination of serological tests, such as SEA IgA and SWAP IgG, to simultaneously establish the diagnosis of schistosomiasis and discriminate the acute from the chronic forms of the disease.
ISSN:0001-706X
1873-6254
DOI:10.1016/j.actatropica.2008.08.012