Follicle-stimulating hormone and testosterone stimulation of immature and mature Sertoli cells in vitro: inhibin and N-cadherin levels and round spermatid binding

• Published in: Journal of andrology 1999-05, Vol.20 (3), p.399-406
• Main Authors: Lampa, J, Hoogerbrugge, J. W, Baarends, W. M, Stanton, P. G, Perryman, K. J, Grootegoed, J. A, Robertson, D. M
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cadherins - metabolism; cell adhesion molecules; Cell Differentiation - drug effects; Cells, Cultured; Dose-Response Relationship, Drug; Follicle Stimulating Hormone - pharmacology; Fundamental and applied biological sciences. Psychology; Hormone metabolism and regulation; Immunohistochemistry; Inhibins - metabolism; Male; Mammalian male genital system; Rats; Rats, Sprague-Dawley; Sertoli Cells - cytology; Sertoli Cells - drug effects; Sertoli Cells - metabolism; Spermatids - metabolism; spermatogenesis; Testis; Testosterone - pharmacology; tissue culture; Vertebrates: reproduction
• ISSN: 0196-3635; 1939-4640
• DOI: 10.1002/j.1939-4640.1999.tb02534.x

The in vitro response of Sertoli cells isolated from adult rat testes to testosterone (T) and follicle‐stimulating hormone (FSH) treatment was investigated. Sertoli cells from >70‐day‐old Sprague‐Dawley rats were isolated by a combined enzymatic treatment followed by the removal of the majority of contaminating germ cells with immobilized peanut agglutinin lectin. Sertoli cells were then cultured for 6–10 days, forming a confluent layer with a cell viability of >83% and 74–77% purity. The contaminating cells were peritubular cells (4–6%), pachytene spermatocytes (4–5%), round spermatids (