Toward a canine model of atopic dermatitis: amplification of cytokine-gene transcripts in the skin of atopic dogs

: The objectives of the present study were to characterize and compare the repertoire of cytokine‐genes transcribed in skin homogenates obtained from normal dogs and dogs with atopic dermatitis (AD) using a reverse‐transcriptase polymerase chain reaction and canine‐specific cytokine‐gene primers. Wh...

Full description

Saved in:
Bibliographic Details
Published in:Experimental dermatology 1999-06, Vol.8 (3), p.204-211
Main Authors: Olivry, T., Dean, G. A., Tompkins, M. B., Dow, J. L., Moore, P. F.
Format: Article
Language:English
Subjects:
Allergic diseases
Animals
atopic dermatitis
Biological and medical sciences
Biopsy
canine
cytokine
Cytokines - genetics
Dermatitis, Atopic - genetics
Dermatitis, Atopic - pathology
Disease Models, Animal
Dogs
Immunopathology
interferon
Interferon-gamma - genetics
interleukin
Interleukin-12 - genetics
Interleukin-2 - genetics
Interleukin-4 - genetics
Interleukin-5 - genetics
Medical sciences
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
skin
Skin - metabolism
Skin - pathology
Skin allergic diseases. Stinging insect allergies
Transcription, Genetic
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:: The objectives of the present study were to characterize and compare the repertoire of cytokine‐genes transcribed in skin homogenates obtained from normal dogs and dogs with atopic dermatitis (AD) using a reverse‐transcriptase polymerase chain reaction and canine‐specific cytokine‐gene primers. Whereas IL‐4 and IL‐5 cytokine‐gene transcripts were detected more commonly in atopic skin biopsy homogenates, IL‐2 mRNA was amplified more often from normal control specimens. IFN‐γ mRNA was detected in 5/29 atopic specimens, 4 of them obtained from the only dog with chronic skin lesions. One‐fourth of atopic samples exhibited clear type‐2 cytokine profiles; the remainder did not demonstrate polarized repertoires. Conversely, type‐1 cytokine profiles were characterized in one‐fourth of normal control specimens. The present study establishes, for the first time, the transcription of type‐2 cytokine‐genes in the skin of dogs with AD. Future experiments investigating the cellular origin and dynamics of allergic cytokine‐gene transcription are needed to confirm whether or not canine AD could be considered an immunological model for a human disease.
ISSN:0906-6705
1600-0625
DOI:10.1111/j.1600-0625.1999.tb00372.x