Detection of erbB-2 Amplifications in Tumors and Sera from Esophageal Carcinoma Patients

We used TaqMan PCR to detect quantitative anomalies of tumor markers in both tumor and serum DNA from esophageal cancer patients. We demonstrated the potential of this methodology by detecting erbB -2 amplifications in a plurality of esophageal tumor samples. These amplifications were corroborated b...

Full description

Saved in:
Bibliographic Details
Published in:Clinical cancer research 1999-06, Vol.5 (6), p.1381-1386
Main Authors: CHIANG, P.-W, BEER, D. G, WEI, W.-L, ORRINGER, M. B, KURNIT, D. M
Format: Article
Language:English
Subjects:
Adenocarcinoma - blood
Adenocarcinoma - genetics
Biological and medical sciences
Blotting, Southern
Cyclin-Dependent Kinase Inhibitor p16 - genetics
DNA, Neoplasm - blood
Esophageal Neoplasms - blood
Esophageal Neoplasms - genetics
Esophagus
Gastroenterology. Liver. Pancreas. Abdomen
Gene Amplification
Gene Dosage
Genes, erbB-2 - genetics
Humans
Medical sciences
Polymerase Chain Reaction
Receptors, Chemokine - genetics
Tumor Suppressor Protein p53 - genetics
Tumors
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We used TaqMan PCR to detect quantitative anomalies of tumor markers in both tumor and serum DNA from esophageal cancer patients. We demonstrated the potential of this methodology by detecting erbB -2 amplifications in a plurality of esophageal tumor samples. These amplifications were corroborated by Southern blots. We then showed the potential of this methodology to detect quantitative anomalies of erbB -2 in serum DNA from individuals with a corresponding amplification in the tumor. The capability of TaqMan PCR to detect abnormalities in serum of esophageal cancer patients creates an opportunity to diagnose esophageal cancer and to monitor the outcome of treatment with a blood test.
ISSN:1078-0432
1557-3265