Identification of phosphate-regulated genes by differential expression in the UV-irradiated host system

The UV-irradiated host system has been used for identifying protein products of genes cloned in a phage vector. By starving the host cells for phosphate immediately before UV-irradiation, we demonstrate that phosphate-regulated genes can be easily identified. By employing this new technique, we also...

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Bibliographic Details
Published in:Journal of microbiological methods 1999-07, Vol.37 (1), p.93-96
Main Authors: Chuang, Shuang-En, Blattner, Frederick R.
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacteriological methods and techniques used in bacteriology
Bacteriology
Bacteriophage lambda
Biological and medical sciences
Culture Media - chemistry
Escherichia coli
Escherichia coli - genetics
Escherichia coli - growth & development
Escherichia coli - radiation effects
Escherichia coli - virology
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Bacterial
Genes, Bacterial
Glycerolphosphate Dehydrogenase - genetics
Heat shock
Lambda
Microbiology
Phosphate starvation
Phosphates
Ultraviolet Rays
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Summary:The UV-irradiated host system has been used for identifying protein products of genes cloned in a phage vector. By starving the host cells for phosphate immediately before UV-irradiation, we demonstrate that phosphate-regulated genes can be easily identified. By employing this new technique, we also provide evidence showing that the gpsA gene might be a new member of the phosphate starvation-inducible ( psi) genes of E. coli.
ISSN:0167-7012
1872-8359
DOI:10.1016/S0167-7012(99)00050-0