Allergen immunotherapy with nanoparticles containing lipopolysaccharide from Brucella ovis

• Published in: European journal of pharmaceutics and biopharmaceutics 2008-11, Vol.70 (3), p.711-717
• Main Authors: Gómez, Sara, Gamazo, Carlos, San Roman, Beatriz, Ferrer, Marta, Sanz, Maria Luisa, Espuelas, Socorro, Irache, Juan M.
• Format: Article
• Language: English
• Subjects: Adjuvant; Adjuvants, Immunologic - administration & dosage; Adjuvants, Immunologic - chemistry; Allergens - administration & dosage; Allergens - chemistry; Allergens - immunology; Allergy; Anaphylaxis - immunology; Anaphylaxis - prevention & control; Animals; Biological and medical sciences; Brucella ovis - immunology; Drug Carriers; Drug Compounding; Female; General pharmacology; Immunization Schedule; Immunoglobulin G - blood; Injections, Intradermal; Interleukin-10 - blood; Lipopolysaccharide; Lipopolysaccharides - administration & dosage; Lipopolysaccharides - chemistry; Lipopolysaccharides - immunology; Maleates - administration & dosage; Maleates - chemistry; Maleates - immunology; Medical sciences; Mice; Mice, Inbred BALB C; Nanoparticles; Ovalbumin; Ovalbumin - administration & dosage; Ovalbumin - chemistry; Ovalbumin - immunology; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polyvinyls - administration & dosage; Polyvinyls - chemistry; Time Factors
• ISSN: 0939-6411; 1873-3441
• DOI: 10.1016/j.ejpb.2008.05.016

The adjuvant and protective capacity against anaphylactic shock of the association between rough lipopolysaccharide of Brucella ovis (LPS) coencapsulated with ovalbumin (OVA), as a model allergen, in Gantrez ® AN nanoparticles was investigated. Several strategies were performed in order to study the adjuvant effect of the LPS either encapsulated or coating the nanoparticles. OVA, as well as LPS, was incorporated either during the manufacturing process (OVA-encapsulated or LPS-encapsulated nanoparticles, respectively) or after the preparation (OVA-coated or LPS-coated nanoparticles, respectively). After the administration of 10 μg of OVA incorporated in the different formulations, all the nanoparticles, with or without LPS, were capable of amplifying the immune response (IgG 1 and IgG 2a). However, in a model of sensitized mice to OVA, the formulation with OVA and LPS-entrapped inside the nanoparticles administered intradermally in three doses of 3 μg of OVA each was the only treatment that totally protected the mice from death after a challenge with an intraperitoneal injection of OVA. In contrast, the control group administered with OVA adsorbed onto a commercial alhydrogel adjuvant showed 80% mortality. These results are highly suggestive for the valuable use of Gantrez ® nanoparticles combined with rough LPS of B. ovis in immunotherapy.