Adipose tissue 11-beta-Hydroxysteroid Dehydrogenase Type 1 and Hexose-6-Phosphate Dehydrogenase gene expressions are increased in patients with type 2 diabetes mellitus

• Published in: Diabetes research and clinical practice 2008-12, Vol.82, p.S135-S140
• Main Authors: Üçkaya, Gökhan, Karadurmuş, Nuri, Kutlu, Onur, Çorakçı, Ahmet, Kızıldağ, Sefa, Ural, Ali Uğur, Gül, Davut, Kutlu, Mustafa
• Format: Article
• Language: English
• Subjects: 11-beta-Hydroxysteroid Dehydrogenase; 11-beta-Hydroxysteroid Dehydrogenase Type 1 - genetics; Adipose tissue; Adipose Tissue - metabolism; Adult; Aged; Carbohydrate Dehydrogenases - genetics; Cortisol metabolism; Diabetes Mellitus, Type 2 - genetics; Endocrinology & Metabolism; Hexose-6-Phosphate Dehydrogenase; Humans; Hypercortisolism; Male; Middle Aged; Polymerase Chain Reaction; Type 2 diabetes mellitus
• ISSN: 0168-8227; 1872-8227
• DOI: 10.1016/j.diabres.2008.09.022

Abstract Aims We have determined 11-beta-Hydroxysteroid Dehydrogenase Type 1 (HSD11B1) and Hexose-6-Phosphate Dehydrogenase (H6PD) mRNA expression levels in adipose tissues from patients with type 2 diabetes mellitus. Methods Six non-diabetic and seven diabetic male patients who undergo elective abdominal surgery were included in the study and visceral and subcutaneous adipose tissue samples were obtained. Fresh preadipocyte cultures were administered to low and high glucose medium (11 M and 25 M) in vitro for 24 h and mRNA extractions were performed. HSD11B1 and H6PD gene mRNA expression levels were determined by real-time PCR and compared. Glyceraldehyde-3-phosphate Dehydrogenase (G3PD) mRNA level is used as housekeeping gene expression. Results HSD11B1 mRNA levels were significantly higher in patient with T2DM than controls in both visceral and subcutaneous adipose tissues (3.35 ± 0.7 vs. 0.37 ± 0.1; P = 0.01 and 2.07 ± 0.8 vs. 0.11 ± 0.05; P = 0.01, respectively). H6PD mRNA levels were also significantly higher in patient with T2DM than controls in both visceral and subcutaneous adipose tissues (3.95 ± 1.2 vs. 1.95 ± 0.8; P = 0.050 and 2.23 ± 1.1 vs. 0.46 ± 0.1; P = 0.043, respectively). Conclusions Failure to down-regulate HSD11B1 activity in patients with type 2 diabetes may contribute to the pathogenesis of T2DM. Subcutaneous and visceral adipose tissues similarly exhibit the same variation in patients with type 2 diabetes mellitus.