Recombinant protein synthesis in Trichoplusia ni BTI-Tn-5B1-4 insect cell aggregates

The Trichoplusia ni BTI‐Tn‐5B1‐4 (Tn‐5B1‐4) insect cell line has received considerable attention as a host for the baculovirus expression vector system. In the present study, suspension cultures were used to compare Tn‐5B1‐4 cell aggregates and cells selected to grow predominantly as individual cell...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology and bioengineering 1999-06, Vol.63 (5), p.612-617
Main Authors: Saarinen, Mark A., Troutner, Kimberly A., Gladden, Steve G., Mitchell-Logean, Christine M., Murhammer, David W.
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - biosynthesis
Alkaline Phosphatase - genetics
Alkaline Phosphatase - metabolism
Animal cell culture
Animal cells
Animals
Baculovirus
baculovirus expression vector system
beta-Galactosidase - biosynthesis
beta-Galactosidase - genetics
beta-Galactosidase - metabolism
Biological and medical sciences
Biotechnology
cell aggregates
Cell Division
Cell Line
Cells
Cloning, Molecular
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Glucose
Glucose - metabolism
insect cells
Insecta
Lactic Acid - metabolism
Methods. Procedures. Technologies
Miscellaneous
Moths - genetics
Noctuidae
Nucleopolyhedrovirus - genetics
Proteins
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Sf-9 cells
Spodoptera frugiperda
Synthesis (chemical)
Trichoplusia ni
Viruses
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Trichoplusia ni BTI‐Tn‐5B1‐4 (Tn‐5B1‐4) insect cell line has received considerable attention as a host for the baculovirus expression vector system. In the present study, suspension cultures were used to compare Tn‐5B1‐4 cell aggregates and cells selected to grow predominantly as individual cells. No significant difference was found between cell aggregates and cells growing predominantly individually in regard to cell growth rate, glucose consumption and lactate accumulation, and specific recombinant protein synthesis levels. In addition, the levels of recombinant protein synthesis were considerably higher than those produced by the commonly used Spodoptera frugiperda Sf‐9 insect cell line. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 63: 612–617, 1999.
ISSN:0006-3592
1097-0290
DOI:10.1002/(SICI)1097-0290(19990605)63:5<612::AID-BIT11>3.0.CO;2-C