Enhanced expression of P-selectin (CD62P) by endothelial cells seeded onto synthetic arterial prostheses (PET, Dacron®) is correlated with leukocyte interactions

• Published in: Journal of biomedical materials research 1999-02, Vol.44 (2), p.156-161
• Main Authors: Guidollet, Jeannine, Chignier, Elza, Pillot, Raymonde, Gayet, Odile, MacGregor, John, Louisot, Pierre
• Format: Article
• Language: English
• Subjects: Adhesion; adhesion molecules; Animals; anti-P-selectin blocking MAb; Batch cell culture; Biocompatible Materials; Biological and medical sciences; Blood; Blood vessel prostheses; Blood Vessel Prosthesis; Cell Adhesion; Cell Communication; Cells; Cells, Cultured; endothelial seeding; Endothelium, Vascular - cytology; Endothelium, Vascular - metabolism; Humans; leukocyte adhesion; Leukocytes - cytology; Leukocytes - metabolism; Materials Testing; Medical sciences; Mice; Neutrophils - cytology; Neutrophils - metabolism; P-selectin (CD62P); P-Selectin - genetics; P-Selectin - metabolism; Polyethylene Terephthalates; Proteins; RNA, Messenger - genetics; RNA, Messenger - metabolism; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; vascular prostheses; Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels
• ISSN: 0021-9304; 1097-4636
• DOI: 10.1002/(SICI)1097-4636(199902)44:2<156::AID-JBM5>3.0.CO;2-H

This study evaluated the expression by seeded endothelial cells (S‐EC) of P‐selectin (CD62P/GMP‐140/PADGEM), an adhesion molecule implicated in endothelial–leukocyte interactions. Endothelial cells were seeded onto knitted polyethylene terephthalate (PET, Dacron®) prostheses and compared with control endothelial cells (C‐EC) cultured in flasks to the same stage. Using flow cytometry techniques, we observed that CD62P expression by PET S‐EC was significantly increased (p < 0.05) compared to C‐EC. Moreover, RT PCR techniques showed that the CD62P RNA level was higher on S‐ECs compared to C‐ECs. Following adhesion assays, increased polymorphonuclear neutrophil (PMN) attachment to the PET‐seeded prostheses as compared to control cultures (p < 0.001) was observed. PMN adherence was enhanced by TNFα activation. PMN adhesion was decreased significantly (p < 0.001) after the incubation of resting EC or TNFα‐activated EC‐seeded prostheses with a blocking monoclonal antibody (LYP20) directed against the P‐selectin. Such results suggest that: (1) PET prosthetic material may induce the expression of P‐selectin by S‐EC; (2) seeding conditions provoke an increase in PMN adhesion; (3) increased PMN interactions with seeded PET material is partially dependent upon P‐selectin expression by the S‐EC. © 1999 John Wiley & Sons, Inc. J Biomed Mater Res, 44, 156–161, 1999.