In vitro development of Staphylococcus aureus biofilms using slime-producing variants and ATP-bioluminescence for automated bacterial quantification

In this work, a method was developed to establish Staphylococcus aureus biofilms on 96‐well plates and automatically quantify viable cells within these biofilms by ATP‐bioluminescence. Different strains were compared for biofilm formation. Cells from slime producing (SP) strain variants were more ad...

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Bibliographic Details
Published in:Luminescence (Chichester, England) England), 1999-01, Vol.14 (1), p.23-31
Main Authors: Gracia, E., Fernández, A., Conchello, P., Alabart, J. L., Pérez, M., Amorena, B.
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - analysis
ATP-bioluminescence
ATP-extractants
Automation
biofilm
Biofilms
Dimethyl Sulfoxide
Genetic Variation
Indicators and Reagents
Luminescence
Photometry - methods
polystyrene support
slime
Space life sciences
Staphylococcus aureus
Staphylococcus aureus - genetics
Staphylococcus aureus - physiology
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Summary:In this work, a method was developed to establish Staphylococcus aureus biofilms on 96‐well plates and automatically quantify viable cells within these biofilms by ATP‐bioluminescence. Different strains were compared for biofilm formation. Cells from slime producing (SP) strain variants were more adherent (p 
ISSN:1522-7235
1522-7243
DOI:10.1002/(SICI)1522-7243(199901/02)14:1<23::AID-BIO513>3.0.CO;2-M