Detection of Borrelia burgdorferi sensu stricto by reverse line blot in the joints of Dutch patients with Lyme arthritis

• Published in: Arthritis and rheumatism 1999-07, Vol.42 (7), p.1473-1480
• Main Authors: Van Der Heijden, Ineke M., Wilbrink, Bert, Rijpkema, Sjoerd G. T., Schouls, Leo M., Heymans, Peter H. M., Van Embden, Jan D. A., Breedveld, Ferdinand C., Tak, Paul P.
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Biological and medical sciences; Biotechnology; Borrelia burgdorferi; Borrelia burgdorferi Group - isolation & purification; Child; Diseases of the osteoarticular system; Female; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; Humans; In vitro gene amplification. Pcr technique; Knee Joint - microbiology; Lyme Disease - epidemiology; Lyme Disease - microbiology; Male; Medical sciences; Methods. Procedures. Technologies; Middle Aged; Netherlands - epidemiology; Nucleic Acid Hybridization; Oligonucleotide Probes - analysis; Osteoarthritis; Random Amplified Polymorphic DNA Technique; Synovial Fluid - microbiology; Synovial Membrane - microbiology
• ISSN: 0004-3591; 1529-0131
• DOI: 10.1002/1529-0131(199907)42:7<1473::AID-ANR22>3.0.CO;2-I

Objective To analyze the presence of Borrelia burgdorferi sensu lato in synovial samples from the knee joint of patients with Lyme arthritis by polymerase chain reaction, and to differentiate the species by reverse line blot (RLB). Methods Synovial fluid (SF) and synovial tissue (ST) samples were obtained from patients with Lyme arthritis (n = 4) and from patients with various other forms of arthritis (n = 9). DNA extracted from synovial samples was amplified by using, as a target, the spacer region between the 5S and 23S ribosomal RNA genes of B burgdorferi sensu lato. Subsequently, 4 species‐specific DNA probes were used in the RLB for specific hybridization. Results DNA from B burgdorferi sensu stricto DNA was detected in the SF and ST from 3 patients with Lyme arthritis. B burgdorferi sensu lato DNA was not detected in the synovial samples from 9 control patients. Conclusion The relationship between different species of B burgdorferi sensu lato and arthritis can be studied using direct analysis of extracted DNA from joint samples. This method can be used to study the association between particular clinical manifestations of Lyme disease and different species of B burgdorferi sensu lato.