Specific Protein Delivery to Target Cells by Antibody-displaying Bionanocapsules

Bionanocapsules (BNCs) are nanoparticles with a high biocompatibility composed of the L protein of the hepatitis B virus surface antigen. BNC can deliver bioactive molecules to hepatocytes efficiently and specifically. However, delivery is limited to hepatocytes and incorporation of proteins into BN...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 2008-12, Vol.144 (6), p.701-707
Main Authors: Kurata, Naoya, Shishido, Takuya, Muraoka, Masaru, Tanaka, Tsutomu, Ogino, Chiaki, Fukuda, Hideki, Kondo, Akihiko
Format: Article
Language:English
Subjects:
Antibodies - chemistry
Antibodies - metabolism
antibody-mediated targeting
bionanocapsule
Drug Carriers - chemistry
Drug Delivery Systems - methods
Genetic Vectors - genetics
Genetic Vectors - metabolism
Green Fluorescent Proteins - chemistry
Green Fluorescent Proteins - metabolism
HeLa Cells
Humans
insect cell expression system
Nanoparticles - chemistry
protein delivery
Protein Engineering - methods
Proteins - administration & dosage
Receptor, Epidermal Growth Factor - chemistry
Receptor, Epidermal Growth Factor - metabolism
ZZ domain
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Summary:Bionanocapsules (BNCs) are nanoparticles with a high biocompatibility composed of the L protein of the hepatitis B virus surface antigen. BNC can deliver bioactive molecules to hepatocytes efficiently and specifically. However, delivery is limited to hepatocytes and incorporation of proteins into BNC is quite troublesome. Here, in order to alter the specificity of BNC and to achieve efficient protein delivery, we developed engineered BNC displaying the ZZ domain of protein A and incorporating enhanced green fluorescent protein (EGFP) inside the particles using an insect cell expression system. The ZZ domain displayed on the surface of BNC binds to anti-epidermal growth factor receptor (EGFR) antibodies, allowing specific delivery of EGFP to HeLa cells. The engineered BNCs are a promising and powerful tool for efficient and cell-specific protein delivery.
ISSN:0021-924X
1756-2651
DOI:10.1093/jb/mvn131