Changes in follicular populations following treatment of buffaloes with PMSG (eCG) and Neutra-PMSG for superovulation

• Published in: Animal reproduction science 1999-05, Vol.56 (1), p.31-38
• Main Authors: Manik, R.S., Singla, S.K., Palta, P., Madan, M.L.
• Format: Article
• Language: English
• Subjects: Animals; BUFALO DE AGUA; Buffalo; Buffaloes - physiology; BUFFLE D'EAU; Chorionic Gonadotropin - pharmacology; Corpus Luteum - diagnostic imaging; Dinoprost - analogs & derivatives; Dinoprost - therapeutic use; eCG; ECHOGRAPHIE; ECHOGRAPHY; ECOGRAFIA; Female; FOLICULOS OVARICOS; Follicle; FOLLICULE OVARIEN; Injections, Intramuscular - veterinary; Injections, Intravenous - veterinary; Neutra-eCG; Ovarian Follicle - growth & development; OVARIAN FOLLICLES; Ovary - diagnostic imaging; Oxytocics - therapeutic use; PMSG; Random Allocation; SUPEROVULACION; SUPEROVULATION; ULTRASON; ULTRASONICS; ULTRASONIDO; Ultrasonography; Videotape Recording; WATER BUFFALOES
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/S0378-4320(99)00026-3

Some 19 buffaloes were synchronized by administration of a prostaglandin (PG) salt Lutalyse, with a single intramuscular (i.m.) injection of 25 mg at day −13. Luteolysis was induced by administration of 50 mg PG, in divided doses of 30 and 20 mg i.m. 12 h apart on day 0 of experiment. The 30 mg PG injection was designated as 0 h of experiment. Group I animals ( n=6) received saline and served as controls while animals in Groups II ( n=7) and III ( n=6) received 2500 I.U. PMSG (eCG) i.m. at day −2. Group III animals were administered 5 ml Neutra-eCG intravenously at 60 h. The number of follicles, classified on the basis of diameter as small (2–5 mm), medium (6–9 mm) and large (≥10 mm) was assessed by ultrasonography on days −2, −1, 0, 1, 2, 5 and 7 of experiment. The number of corpora lutea (CL) was recorded by palpation per rectum on day 8. The number of small follicles which did not differ among the three groups on days 0, 1 and 2 was significantly lower ( P