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Simple blood-spot sampling with nested polymerase chain reaction detection for epidemiology studies on Brugia malayi

In the absence of a suitable Brugia malayi antigen detection assay, PCR remains one of the more sensitive alternatives to Giemsa-stained thick blood films for B. malayi detection. The need for refrigerated storage and transportation of blood has limited the use of PCR for large-scale epidemiology st...

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Bibliographic Details
Published in:International journal for parasitology 1999-05, Vol.29 (5), p.717-721
Main Authors: Cox-Singh, Janet, Pomrehn, Andrea S, Rahman, Hasan A, Zakaria, Robaiza, Miller, Andy O, Singh, Balbir
Format: Article
Language:English
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Summary:In the absence of a suitable Brugia malayi antigen detection assay, PCR remains one of the more sensitive alternatives to Giemsa-stained thick blood films for B. malayi detection. The need for refrigerated storage and transportation of blood has limited the use of PCR for large-scale epidemiology studies in remote endemic areas. Here we report simple finger-prick blood-spot collection, a one-tube DNA template extraction method and the development of a B. malayi-specific nested PCR assay. The assay was tested on 145 field samples and was positive for all 30 microscopy-positive samples and for an additional 13 samples which were microscopy-negative.
ISSN:0020-7519
1879-0135
DOI:10.1016/S0020-7519(99)00015-6