Molecular Dynamics of Labeled Oligonucleotide Probes Used for the Detection of Point Mutations in DNA

The fluorescent label BODIPY 576/589 linked to the 5′-end of an oligonucleotide via alkyl chain linkers can be used as a probe to detect point mutations in DNA 1 . We have employed fluorescence anisotropy decay and dynamic fluorescence resonance energy transfer (FRET) in order to investigate the mol...

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Bibliographic Details
Published in:Nucleosides & nucleotides 1999-03, Vol.18 (3), p.411-423
Main Authors: Winter, S., Kirschstein, S., Millar, D. P.
Format: Article
Language:English
Subjects:
Base Pair Mismatch
Base Sequence
Boron Compounds
DNA - chemistry
DNA - genetics
Energy Transfer
Fluorescence Polarization
Fluorescent Dyes
Genetic Techniques
Oligonucleotide Probes - chemistry
Point Mutation
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Summary:The fluorescent label BODIPY 576/589 linked to the 5′-end of an oligonucleotide via alkyl chain linkers can be used as a probe to detect point mutations in DNA 1 . We have employed fluorescence anisotropy decay and dynamic fluorescence resonance energy transfer (FRET) in order to investigate the molecular origin of the fluorescence lifetime aberrations of BODIPY in the presence of a mismatched base. The results show that both, an increased flexibility of the alcyl chain linker to the BODIPY molecule, as well as relaxation of the secondary structure of the whole complex, contribute to the decreased fluorescence lifetimes reported previously.
ISSN:0732-8311
DOI:10.1080/15257779908043086