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Molecular Dynamics of Labeled Oligonucleotide Probes Used for the Detection of Point Mutations in DNA
The fluorescent label BODIPY 576/589 linked to the 5′-end of an oligonucleotide via alkyl chain linkers can be used as a probe to detect point mutations in DNA 1 . We have employed fluorescence anisotropy decay and dynamic fluorescence resonance energy transfer (FRET) in order to investigate the mol...
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| Published in: | Nucleosides & nucleotides 1999-03, Vol.18 (3), p.411-423 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c350t-e9a1c4f90b8c757628d6176bbfbe5d76911d8ca65ab8739ed0453dbd35dd34a33 |
| container_end_page | 423 |
| container_issue | 3 |
| container_start_page | 411 |
| container_title | Nucleosides & nucleotides |
| container_volume | 18 |
| creator | Winter, S. Kirschstein, S. Millar, D. P. |
| description | The fluorescent label BODIPY 576/589 linked to the 5′-end of an oligonucleotide via alkyl chain linkers can be used as a probe to detect point mutations in DNA
1
. We have employed fluorescence anisotropy decay and dynamic fluorescence resonance energy transfer (FRET) in order to investigate the molecular origin of the fluorescence lifetime aberrations of BODIPY in the presence of a mismatched base. The results show that both, an increased flexibility of the alcyl chain linker to the BODIPY molecule, as well as relaxation of the secondary structure of the whole complex, contribute to the decreased fluorescence lifetimes reported previously. |
| doi_str_mv | 10.1080/15257779908043086 |
| format | article |
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1
. We have employed fluorescence anisotropy decay and dynamic fluorescence resonance energy transfer (FRET) in order to investigate the molecular origin of the fluorescence lifetime aberrations of BODIPY in the presence of a mismatched base. The results show that both, an increased flexibility of the alcyl chain linker to the BODIPY molecule, as well as relaxation of the secondary structure of the whole complex, contribute to the decreased fluorescence lifetimes reported previously.</description><identifier>ISSN: 0732-8311</identifier><identifier>DOI: 10.1080/15257779908043086</identifier><identifier>PMID: 10408923</identifier><language>eng</language><publisher>United States: Taylor & Francis Group</publisher><subject>Base Pair Mismatch ; Base Sequence ; Boron Compounds ; DNA - chemistry ; DNA - genetics ; Energy Transfer ; Fluorescence Polarization ; Fluorescent Dyes ; Genetic Techniques ; Oligonucleotide Probes - chemistry ; Point Mutation</subject><ispartof>Nucleosides & nucleotides, 1999-03, Vol.18 (3), p.411-423</ispartof><rights>Copyright Taylor & Francis Group, LLC 1999</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c350t-e9a1c4f90b8c757628d6176bbfbe5d76911d8ca65ab8739ed0453dbd35dd34a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10408923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Winter, S.</creatorcontrib><creatorcontrib>Kirschstein, S.</creatorcontrib><creatorcontrib>Millar, D. P.</creatorcontrib><title>Molecular Dynamics of Labeled Oligonucleotide Probes Used for the Detection of Point Mutations in DNA</title><title>Nucleosides & nucleotides</title><addtitle>Nucleosides Nucleotides</addtitle><description>The fluorescent label BODIPY 576/589 linked to the 5′-end of an oligonucleotide via alkyl chain linkers can be used as a probe to detect point mutations in DNA
1
. We have employed fluorescence anisotropy decay and dynamic fluorescence resonance energy transfer (FRET) in order to investigate the molecular origin of the fluorescence lifetime aberrations of BODIPY in the presence of a mismatched base. The results show that both, an increased flexibility of the alcyl chain linker to the BODIPY molecule, as well as relaxation of the secondary structure of the whole complex, contribute to the decreased fluorescence lifetimes reported previously.</description><subject>Base Pair Mismatch</subject><subject>Base Sequence</subject><subject>Boron Compounds</subject><subject>DNA - chemistry</subject><subject>DNA - genetics</subject><subject>Energy Transfer</subject><subject>Fluorescence Polarization</subject><subject>Fluorescent Dyes</subject><subject>Genetic Techniques</subject><subject>Oligonucleotide Probes - chemistry</subject><subject>Point Mutation</subject><issn>0732-8311</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkD1PHDEQhl0kAgL8gDSRK7oL9nq9tqU0iCMQ6YAruNryx2xw5F2D7RW5f8-ejgIJKalGM_M-b_Eg9JWS75RIck55w4UQSs1Ly4jsPqEjIlizkIzSQ_SllD-E8Eap9gAdUtISqRp2hOA2RXBTNBkvt6MZgis49XhlLETw-D6G32mcXIRUgwe8zslCwZsy__qUcX0EvIQKroY07sB1CmPFt1M1u0vBYcTLu4sT9Lk3scDp2zxGm59XD5c3i9X99a_Li9XCMU7qApShru0VsdIJLrpG-o6KztreAveiU5R66UzHjZWCKfCk5cxbz7j3rDWMHaOzfe9TTs8TlKqHUBzEaEZIU9GdkpLPFuYg3QddTqVk6PVTDoPJW02J3vnUH3zOzLe38skO4N8Re5lz4Mc-EMbZzWBeUo5eV7ONKffZjC4Uzf7VL_6Lf6B0_VvZK45dmAU</recordid><startdate>199903</startdate><enddate>199903</enddate><creator>Winter, S.</creator><creator>Kirschstein, S.</creator><creator>Millar, D. P.</creator><general>Taylor & Francis Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199903</creationdate><title>Molecular Dynamics of Labeled Oligonucleotide Probes Used for the Detection of Point Mutations in DNA</title><author>Winter, S. ; Kirschstein, S. ; Millar, D. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c350t-e9a1c4f90b8c757628d6176bbfbe5d76911d8ca65ab8739ed0453dbd35dd34a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Base Pair Mismatch</topic><topic>Base Sequence</topic><topic>Boron Compounds</topic><topic>DNA - chemistry</topic><topic>DNA - genetics</topic><topic>Energy Transfer</topic><topic>Fluorescence Polarization</topic><topic>Fluorescent Dyes</topic><topic>Genetic Techniques</topic><topic>Oligonucleotide Probes - chemistry</topic><topic>Point Mutation</topic><toplevel>online_resources</toplevel><creatorcontrib>Winter, S.</creatorcontrib><creatorcontrib>Kirschstein, S.</creatorcontrib><creatorcontrib>Millar, D. P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nucleosides & nucleotides</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Winter, S.</au><au>Kirschstein, S.</au><au>Millar, D. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Dynamics of Labeled Oligonucleotide Probes Used for the Detection of Point Mutations in DNA</atitle><jtitle>Nucleosides & nucleotides</jtitle><addtitle>Nucleosides Nucleotides</addtitle><date>1999-03</date><risdate>1999</risdate><volume>18</volume><issue>3</issue><spage>411</spage><epage>423</epage><pages>411-423</pages><issn>0732-8311</issn><abstract>The fluorescent label BODIPY 576/589 linked to the 5′-end of an oligonucleotide via alkyl chain linkers can be used as a probe to detect point mutations in DNA
1
. We have employed fluorescence anisotropy decay and dynamic fluorescence resonance energy transfer (FRET) in order to investigate the molecular origin of the fluorescence lifetime aberrations of BODIPY in the presence of a mismatched base. The results show that both, an increased flexibility of the alcyl chain linker to the BODIPY molecule, as well as relaxation of the secondary structure of the whole complex, contribute to the decreased fluorescence lifetimes reported previously.</abstract><cop>United States</cop><pub>Taylor & Francis Group</pub><pmid>10408923</pmid><doi>10.1080/15257779908043086</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0732-8311 |
| ispartof | Nucleosides & nucleotides, 1999-03, Vol.18 (3), p.411-423 |
| issn | 0732-8311 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69885052 |
| source | Taylor and Francis Science and Technology Collection |
| subjects | Base Pair Mismatch Base Sequence Boron Compounds DNA - chemistry DNA - genetics Energy Transfer Fluorescence Polarization Fluorescent Dyes Genetic Techniques Oligonucleotide Probes - chemistry Point Mutation |
| title | Molecular Dynamics of Labeled Oligonucleotide Probes Used for the Detection of Point Mutations in DNA |
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