Assay for levormeloxifene, a selective estrogen receptor modulator, in human and monkey plasma employing high-performance liquid chromatography and solid-phase extraction

Assays for levormeloxifene, a new selective estrogen receptor modulator, and its 7-desmethyl metabolite in human and cynomolgus monkey plasma are described. Plasma was extracted on mixed-mode bonded sorbent material (C 8/SCX) and the extracts were analysed by high-performance liquid chromatography w...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1999-06, Vol.729 (1), p.315-322
Main Authors: Kiehr, Benedicte, Christensen, Michael S
Format: Article
Language:English
Subjects:
Analysis
Animals
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
General pharmacology
Humans
Levormeloxifene
Macaca fascicularis
Medical sciences
Pharmacology. Drug treatments
Pyrrolidines - blood
Receptors, Estrogen - drug effects
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Fluorescence
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Summary:Assays for levormeloxifene, a new selective estrogen receptor modulator, and its 7-desmethyl metabolite in human and cynomolgus monkey plasma are described. Plasma was extracted on mixed-mode bonded sorbent material (C 8/SCX) and the extracts were analysed by high-performance liquid chromatography with fluorescence detection. Recoveries of levormeloxifene and the metabolite exceeded 70%. Within and total assay precision calculated as a coefficient of variation (C.V.) were
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(99)00179-6