Molecular cloning and characterization of a cDNA encoding the human leucocyte vacuolar protein sorting (hlVps45)

• Published in: The international journal of biochemistry & cell biology 1999-06, Vol.31 (6), p.683-694
• Main Authors: Rajasekariah, Poornima, Eyre, Helen J, Stanley, Keith K, Walls, Ronald S, Sutherland, Grant R
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Base Sequence; Biological Transport; Carrier Proteins - chemistry; Carrier Proteins - genetics; Chromosome localization; Chromosome Mapping; Chromosomes, Human, Pair 1; Cloning, Molecular; DNA, Complementary - chemistry; Golgi Apparatus - metabolism; Humans; Leukocytes - chemistry; Membrane Proteins - chemistry; Membrane Proteins - genetics; Molecular Sequence Data; mRNA expression; Nucleotide sequencing; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Sequence Alignment; Syntaxin; Vesicle transport; Vesicular Transport Proteins
• ISSN: 1357-2725; 1878-5875
• DOI: 10.1016/S1357-2725(99)00017-5

We have isolated a novel cDNA clone from human leucocyte cDNA library, encoding a Sec1p-like vacuolar protein sorting (hl Vps45) which is believed to be implicated in vesicular transportation. Although the deduced amino acid (AA) sequence of this cDNA has revealed 97% identity to other known mammalian vacuolar protein sorting, there is an extensive variation in nucleotide sequence in comparison to that of three previously reported human (h Vps45), rat (r Vps45) and mouse (m Vps45) vacuolar protein sorting ( Vps45) cDNAs [ 1–3]. At the nucleotide sequence level hl Vps45 demonstrated 90% homology to the h Vps45 and r Vps45 and 89% identity to m Vps45 with no significant homology in their noncoding regions. The 2.4 Kb mRNA corresponding to the hl Vps45 clone is widely distributed in a variety of human tissues expressing highest levels in peripheral blood mononuclear cells (PBMC), neutrophils, heart, spleen, and testis. The chromosomal mapping studies have demonstrated that the hl Vps45 is localized to long arm of human chromosome 1 at q21–q22. Our data indicates that we have isolated, characterized and mapped a novel cDNA encoding hl Vps45, which may play an important role in protein trafficking as well as have clinical significance in the release of inflammatory mediators e.g. histamine, bradykinin and cytokine release.