Immunohistochemical analysis of hepatic interferon alpha-beta receptor level: relationship between receptor expression and response to interferon therapy in patients with chronic hepatitis C

Background/Aims: This study aimed to determine the expression level of interferon alpha/beta (IFN-α/β) receptor in the liver immunohistochemically and evaluate its usefulness in predicting the outcome to IFN therapy in patients with chronic hepatitis C. Methods: The level of IFN-α/β receptor express...

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Bibliographic Details
Published in:Journal of hepatology 1999-06, Vol.30 (6), p.995-1003
Main Authors: Yatsuhashi, Hiroshi, Fujino, Tatsuya, Matsumoto, Takenori, Inoue, Osami, Koga, Michiaki, Yano, Michitami
Format: Article
Language:English
Subjects:
Adult
Aged
Biological and medical sciences
Chronic hepatitis C
Female
HCV-RNA levels
Hepacivirus - genetics
Hepatitis A - immunology
Hepatitis C, Chronic - immunology
Hepatitis C, Chronic - therapy
Hepatitis C, Chronic - virology
Humans
Immunohistochemistry
Immunomodulators
Interferon
Interferon α/β receptor
Interferon-alpha - therapeutic use
Liver - chemistry
Male
Medical sciences
Membrane Proteins
Middle Aged
Pharmacology. Drug treatments
Receptor, Interferon alpha-beta
Receptors, Interferon - analysis
RNA, Viral - blood
Viremia - therapy
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Summary:Background/Aims: This study aimed to determine the expression level of interferon alpha/beta (IFN-α/β) receptor in the liver immunohistochemically and evaluate its usefulness in predicting the outcome to IFN therapy in patients with chronic hepatitis C. Methods: The level of IFN-α/β receptor expression was determined in immunoperoxidase-stained pretreatment sections of 55 chronic hepatitis C patients later treated with IFN. We used liver biopsy specimens and mouse monoclonal anti-human IFN-α/β receptor antibody. Quantitative analysis of immunostaining was performed by image analysis software. The level of IFN-α/β receptor was expressed as Unit (U). Sustained responders were patients who showed persistent disappearance of serum HCV-RNA during the 6-month period after treatment, while non-responders showed persistence of viremia after therapy. Results: Positive immunostaining was observed in the cytoplasm of hepatocytes. The mean expression level of hepatic IFN-α/β receptor in sustained responders (2.65±1.11 U, n=15) was significantly ( p
ISSN:0168-8278
1600-0641
DOI:10.1016/S0168-8278(99)80252-9