cAMP-Dependent Positive Control of Cyclin A2 Expression during G1/S Transition in Primary Hepatocytes

cAMP positively and negatively regulates hepatocyte proliferation but its molecular targets are still unknown. Cyclin A2 is a major regulator of the cell cycle progression and its synthesis is required for progression to S phase. We have investigated whether cyclin A2 and cyclin A2-associated kinase...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1999-07, Vol.261 (1), p.118-122
Main Authors: Desdouets, Chantal, Thoresen, G.Hege, Senamaud-Beaufort, Catherine, Christoffersen, Thoralf, Brechot, Christian, Sobczak-Thepot, Joëlle
Format: Article
Language:English
Subjects:
Animals
Blotting, Western
cell cycle
Cell Division - drug effects
Cells, Cultured
cyclic AMP
Cyclic AMP - metabolism
Cyclin A - metabolism
Cyclin A2
Cyclin-Dependent Kinases - metabolism
Cyclins - metabolism
DNA - biosynthesis
DNA synthesis
G1 Phase - drug effects
Glucagon - pharmacology
hepatocytes
Liver - cytology
Liver - drug effects
Liver - metabolism
Male
Rats
Rats, Wistar
S Phase - drug effects
Signal Transduction - drug effects
Time Factors
Up-Regulation - drug effects
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Summary:cAMP positively and negatively regulates hepatocyte proliferation but its molecular targets are still unknown. Cyclin A2 is a major regulator of the cell cycle progression and its synthesis is required for progression to S phase. We have investigated whether cyclin A2 and cyclin A2-associated kinase might be one of the targets for the cAMP transduction pathway during progression of hepatocytes through G1 and G1/S. We show that stimulation of primary cultured hepatocytes by glucagon differentially modulated the expression of G1/S cyclins. Glucagon indeed upregulated cyclin A2 and cyclin A2-associated kinase while cyclin E-associated kinase was unmodified. In conclusion, our study identifies cyclin A2 as an important effector of the cAMP transduction network during hepatocyte proliferation.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1999.0575