Determination of acid α-naphthyl acetate esterase enzyme activity in peripheral blood leukocytes of gazelles (Gazella subgutturosa)

We examined gazelle peripheral blood leucocytes using the α-Naphth y l acetate esterase (ANAE) staining technique (pH 5.8). Our purpose was to determine the percentage of ANAE positive lymphocytes. The proportion of ANAE positive T-lymphocytes was 72%. T-lymphocytes showed an ANAE positive reaction,...

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Bibliographic Details
Published in:Biotechnic & histochemistry 2008-01, Vol.83 (6), p.279-283
Main Authors: Altunay, H, Harem, Iş, Harem, MK, Aştı, RN, Kurtdede, N
Format: Article
Language:English
Subjects:
ANAE
Animals
Blood Platelets - enzymology
gazelle
Leukocytes - enzymology
Naphthol AS D Esterase - analysis
Naphthol AS D Esterase - metabolism
peripheral blood
Ruminants - blood
α-naphthyl acetate asterase
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Summary:We examined gazelle peripheral blood leucocytes using the α-Naphth y l acetate esterase (ANAE) staining technique (pH 5.8). Our purpose was to determine the percentage of ANAE positive lymphocytes. The proportion of ANAE positive T-lymphocytes was 72%. T-lymphocytes showed an ANAE positive reaction, but eosinophilic granulocytes and monocytes also showed a positive reaction. By contrast, no reaction was detected in B-lymphocytes, neutrophil granulocytes or platelets. The reaction observed in T-lymphocytes was a red-brown coloration, usually 1-2 granules, but enough granules to fill the cytoplasm were detected rarely. As a result of ANAE enzyme staining, we concluded that the staining technique can be used as a cytochemical marker for gazelle T-lymphocytes.
ISSN:1052-0295
1473-7760
DOI:10.1080/10520290802602075