Increased Vulnerability of NFH-LacZ Transgenic Mouse to Traumatic Brain Injury-Induced Behavioral Deficits and Cortical Damage

• Published in: Journal of cerebral blood flow and metabolism 1999-07, Vol.19 (7), p.762-770
• Main Authors: Nakamura, Michio, Saatman, Kathryn E., Galvin, James E., Scherbel, Uwe, Raghupathi, Ramesh, Trojanowski, John Q., McIntosh, Tracy K.
• Format: Article
• Language: English
• Subjects: Animals; Behavior, Animal; Biological and medical sciences; Brain Injuries - genetics; Brain Injuries - physiopathology; Cerebral Cortex - pathology; Cerebral Cortex - physiopathology; Gene Expression Regulation; Injuries of the nervous system and the skull. Diseases due to physical agents; Lac Operon; Medical sciences; Mice; Mice, Transgenic; Neurofilament Proteins - physiology; Recombinant Fusion Proteins - physiology; Traumas. Diseases due to physical agents
• ISSN: 0271-678X; 1559-7016
• DOI: 10.1097/00004647-199907000-00006

The authors evaluated the neurobehavioral and neuropathologic sequelae after traumatic brain injury (TBI) in transgenic (TG) mice expressing truncated high molecular weight neurofilament (NF) protein fused to beta-galactosidase (NFH-LacZ), which develop Lewy body-like NF-rich inclusions throughout the CNS. TG mice and their wild-type (WT) littermates were subjected to controlled cortical impact brain injury (TG, n=19; WT, n=17) or served as uninjured controls (TG, n =11; WT, n =11). During a 3-week period, mice were evaluated with an array of neuromotor function tests including neuroscore, beam balance, and both fast and slow acceleration rotarod. Brain-injured WT and TG mice showed significant motor dysfunction until 15 days and 21 days post-injury, respectively (P < .025). Compared with brain-injured WT mice, brain-injured TG mice had significantly greater motor dysfunction as assessed by neuroscore (P < .01) up to and including 15 days post-injury. Similarly, brain-injured TG mice performed significantly worse than brain-injured WT mice on slow acceleration rotarod at 2, 8, and 15 days post-injury (P < .05), and beam balance over 2 weeks post-injury (P < .01). Histopathologic analysis showed significantly greater tissue loss in the injured hemisphere in TG mice at 4 weeks post-injury (P < .01). Together these data show that NFH-LacZ TG mice are more behaviorally and histologically vulnerable to TBI than WT mice, suggesting that the presence of NF-rich inclusions may exacerbate neuromotor dysfunction and cell death after TBI.