CD4 TCRBV CDR3 Analysis in prevalent SLE Cases from two ethnic Groups

We examined CD4+ T cell TCRBV-CDR3 transcripts from 19 lupus patients and 16 controls to test the hypothesis that CD4+ TCRBV-CDR3 expression in SLE differs from normals. Within the disease group we also performed exploratory analyses to determine the association between risk of oligoclonality and HL...

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Bibliographic Details
Published in:Lupus 1999-01, Vol.8 (4), p.311-319
Main Authors: Fraser, P A, Lu, L-Y, DeCeulaer, K, Schur, P H, Fici, D, Awdeh, Z, Ding, W-Z, Levitan, E, Lew, R, Uko, G, Gonzalez, C
Format: Article
Language:English
Subjects:
Adult
African Continental Ancestry Group
Age
Aged
Antibodies, Antinuclear - blood
Autoimmune diseases
CD3 Complex - analysis
CD4-Positive T-Lymphocytes - chemistry
CD4-Positive T-Lymphocytes - immunology
DNA - immunology
DNA Primers
European Continental Ancestry Group
Female
Flow Cytometry
Genes
Histocompatibility Testing
HLA-DR Antigens - analysis
HLA-DRB1 Chains
HLA-DRB3 Chains
HLA-DRB4 Chains
HLA-DRB5 Chains
Hospitals
Humans
Hypotheses
Lupus
Lupus Erythematosus, Systemic - ethnology
Lupus Erythematosus, Systemic - immunology
Lymphocytes
Middle Aged
Patients
Peptides
Phlebotomy
Prevalence
Receptors, Antigen, T-Cell, alpha-beta - immunology
Rheumatoid arthritis
Serology
T cell receptors
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Summary:We examined CD4+ T cell TCRBV-CDR3 transcripts from 19 lupus patients and 16 controls to test the hypothesis that CD4+ TCRBV-CDR3 expression in SLE differs from normals. Within the disease group we also performed exploratory analyses to determine the association between risk of oligoclonality and HLA-DRB specificities and the duration of the CDR3 patterns. Oligoclonal patterns consistent with CDR3 restriction were three times more likely in SLE than in controls (OR=3.7). TCRBV1, BV4, BV5.1, BV7, BV9, BV18 and BV22 gene segment CDR3 patterns of oligoclonality were seenexclusively amonglupus patients. HLA-DRB3increasedtherisk ofoligoclonalexpressionin SLE. In four patients studied over time, the pattern of TCRBV-CDR3 expression was stable in a second sample obtained 6–14 months later. The increased frequency of CD4+ T cell TCRBV-CDR3 oligoclonal expression in SLE when compared to controls and the persistence of these patterns are consistent with an expanded pool of autoreactive CD4 T cells in SLE which recognize peptides derived from autoantigens. The association of HLA-DRB3 genes with increased risk of CDR3 oligoclonality among the SLE subjects is compatible with the hypothesis that molecules encoded by HLA-DRB3 may facilitate autoantigen recognition by CD4 T cells.
ISSN:0961-2033
1477-0962
DOI:10.1191/096120399678847902