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Diversity studies of Azotobacter spp. from cotton-wheat cropping systems of India
Azotobacter are the favored bioinoculants being promoted for cotton crop in India. In order to develop bioinoculants, both metabolic fingerprinting and genetic fingerprinting have been used to study the diversity among Azotobacter spp. isolated from four different cotton-wheat cropping regions of In...
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| Published in: | Journal of basic microbiology 2008-12, Vol.48 (6), p.455-463 |
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| Main Authors: | , , |
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| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c4459-ef66b8cd0c0f8098f085a959e5fa411172096ea2b94f00382845bd7dd139538c3 |
| container_end_page | 463 |
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| container_title | Journal of basic microbiology |
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| creator | Bhatia, Ranjana Ruppel, Silke Narula, Neeru |
| description | Azotobacter are the favored bioinoculants being promoted for cotton crop in India. In order to develop bioinoculants, both metabolic fingerprinting and genetic fingerprinting have been used to study the diversity among Azotobacter spp. isolated from four different cotton-wheat cropping regions of India. On the basis of acetylene reduction, indole acetic acid production and ammonia excretion, from 76 free-living diazotrophs isolated from the rhizospheric soil of cotton, 20 efficient isolates were selected for further studies. Morphological characterization indicated a close resemblance of these isolates to Azotobacter spp. BIOLOG cataloguing divided them into two main groups, but amplified ribosomal DNA restriction analysis clustered the isolates from the four regions having different soil types into four separate sub-clusters. Metabolic fingerprinting was not able to detect subtle differences among the isolates as achieved with genetic fingerprinting. However, 16S rRNA is a highly conserved locus. Variations observed could be due to domestication of the isolates in different agro-ecological niches. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim) |
| doi_str_mv | 10.1002/jobm.200800059 |
| format | article |
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In order to develop bioinoculants, both metabolic fingerprinting and genetic fingerprinting have been used to study the diversity among Azotobacter spp. isolated from four different cotton-wheat cropping regions of India. On the basis of acetylene reduction, indole acetic acid production and ammonia excretion, from 76 free-living diazotrophs isolated from the rhizospheric soil of cotton, 20 efficient isolates were selected for further studies. Morphological characterization indicated a close resemblance of these isolates to Azotobacter spp. BIOLOG cataloguing divided them into two main groups, but amplified ribosomal DNA restriction analysis clustered the isolates from the four regions having different soil types into four separate sub-clusters. Metabolic fingerprinting was not able to detect subtle differences among the isolates as achieved with genetic fingerprinting. However, 16S rRNA is a highly conserved locus. 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KGaA, Weinheim)</description><identifier>ISSN: 0233-111X</identifier><identifier>EISSN: 1521-4028</identifier><identifier>DOI: 10.1002/jobm.200800059</identifier><identifier>PMID: 18785658</identifier><language>eng</language><publisher>Weinheim: Wiley-VCH Verlag</publisher><subject>16S rRNA gene ; Ammonia excretion ; Azotobacter ; Azotobacter - classification ; Azotobacter - genetics ; Azotobacter - isolation & purification ; BIOLOG ; Cotton-wheat cropping systems ; DNA Fingerprinting ; DNA, Bacterial - genetics ; DNA, Bacterial - isolation & purification ; DNA, Ribosomal - genetics ; DNA, Ribosomal - isolation & purification ; Genetic Variation ; Gossypium - genetics ; Gossypium - microbiology ; India ; Indole acetic acid ; Nitrogenase assay ; RNA, Ribosomal, 16S - genetics ; Soil Microbiology ; Triticum - genetics ; Triticum - microbiology</subject><ispartof>Journal of basic microbiology, 2008-12, Vol.48 (6), p.455-463</ispartof><rights>Copyright © 2008 WILEY‐VCH Verlag GmbH & Co. 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Basic Microbiol</addtitle><description>Azotobacter are the favored bioinoculants being promoted for cotton crop in India. In order to develop bioinoculants, both metabolic fingerprinting and genetic fingerprinting have been used to study the diversity among Azotobacter spp. isolated from four different cotton-wheat cropping regions of India. On the basis of acetylene reduction, indole acetic acid production and ammonia excretion, from 76 free-living diazotrophs isolated from the rhizospheric soil of cotton, 20 efficient isolates were selected for further studies. Morphological characterization indicated a close resemblance of these isolates to Azotobacter spp. BIOLOG cataloguing divided them into two main groups, but amplified ribosomal DNA restriction analysis clustered the isolates from the four regions having different soil types into four separate sub-clusters. Metabolic fingerprinting was not able to detect subtle differences among the isolates as achieved with genetic fingerprinting. However, 16S rRNA is a highly conserved locus. Variations observed could be due to domestication of the isolates in different agro-ecological niches. (© 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)</description><subject>16S rRNA gene</subject><subject>Ammonia excretion</subject><subject>Azotobacter</subject><subject>Azotobacter - classification</subject><subject>Azotobacter - genetics</subject><subject>Azotobacter - isolation & purification</subject><subject>BIOLOG</subject><subject>Cotton-wheat cropping systems</subject><subject>DNA Fingerprinting</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>DNA, Ribosomal - genetics</subject><subject>DNA, Ribosomal - isolation & purification</subject><subject>Genetic Variation</subject><subject>Gossypium - genetics</subject><subject>Gossypium - microbiology</subject><subject>India</subject><subject>Indole acetic acid</subject><subject>Nitrogenase assay</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Soil Microbiology</subject><subject>Triticum - genetics</subject><subject>Triticum - microbiology</subject><issn>0233-111X</issn><issn>1521-4028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><recordid>eNqFkM1P2zAYh60JNErHdUfIabd0r-04sY9AB2N8CUG3iYvlJDaYNXVmu4Py15OSqnDb6b08v0evHoQ-YxhhAPL1wZXNiABwAGDiAxpgRnCaAeEbaACE0hRj_HsLbYfw0CFCEPERbWFecJYzPkBXY_tP-2DjIglxXlsdEmeS_WcXXamqqH0S2naUGO-apHIxuln6eK9VTCrv2tbO7pKwCFE3r7OTWW3VJ7Rp1DTondUdosnRt5vD7-nZ5fHJ4f5ZWmUZE6k2eV7yqoYKDAfBDXCmBBOaGZV1PxcERK4VKUVmACgnPGNlXdQ1poJRXtEh-tJ7W-_-znWIsrGh0tOpmmk3DzIXXAjGcAeOerB7OQSvjWy9bZRfSAxyGVEuI8p1xG6wuzLPy0bXb_iqWgeIHni0U734j07-uDw4fy9P-63tsj2tt8r_kXlBCyZ_XRxLwn4enJ6Px_K24_d63ign1Z23QU6uCWAKmBUkzwR9ATlrlrQ</recordid><startdate>200812</startdate><enddate>200812</enddate><creator>Bhatia, Ranjana</creator><creator>Ruppel, Silke</creator><creator>Narula, Neeru</creator><general>Wiley-VCH Verlag</general><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200812</creationdate><title>Diversity studies of Azotobacter spp. from cotton-wheat cropping systems of India</title><author>Bhatia, Ranjana ; Ruppel, Silke ; Narula, Neeru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4459-ef66b8cd0c0f8098f085a959e5fa411172096ea2b94f00382845bd7dd139538c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>16S rRNA gene</topic><topic>Ammonia excretion</topic><topic>Azotobacter</topic><topic>Azotobacter - classification</topic><topic>Azotobacter - genetics</topic><topic>Azotobacter - isolation & purification</topic><topic>BIOLOG</topic><topic>Cotton-wheat cropping systems</topic><topic>DNA Fingerprinting</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>DNA, Ribosomal - genetics</topic><topic>DNA, Ribosomal - isolation & purification</topic><topic>Genetic Variation</topic><topic>Gossypium - genetics</topic><topic>Gossypium - microbiology</topic><topic>India</topic><topic>Indole acetic acid</topic><topic>Nitrogenase assay</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Soil Microbiology</topic><topic>Triticum - genetics</topic><topic>Triticum - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bhatia, Ranjana</creatorcontrib><creatorcontrib>Ruppel, Silke</creatorcontrib><creatorcontrib>Narula, Neeru</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of basic microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bhatia, Ranjana</au><au>Ruppel, Silke</au><au>Narula, Neeru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diversity studies of Azotobacter spp. from cotton-wheat cropping systems of India</atitle><jtitle>Journal of basic microbiology</jtitle><addtitle>J. Basic Microbiol</addtitle><date>2008-12</date><risdate>2008</risdate><volume>48</volume><issue>6</issue><spage>455</spage><epage>463</epage><pages>455-463</pages><issn>0233-111X</issn><eissn>1521-4028</eissn><abstract>Azotobacter are the favored bioinoculants being promoted for cotton crop in India. In order to develop bioinoculants, both metabolic fingerprinting and genetic fingerprinting have been used to study the diversity among Azotobacter spp. isolated from four different cotton-wheat cropping regions of India. On the basis of acetylene reduction, indole acetic acid production and ammonia excretion, from 76 free-living diazotrophs isolated from the rhizospheric soil of cotton, 20 efficient isolates were selected for further studies. 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| ispartof | Journal of basic microbiology, 2008-12, Vol.48 (6), p.455-463 |
| issn | 0233-111X 1521-4028 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69899551 |
| source | Wiley-Blackwell Read & Publish Collection |
| subjects | 16S rRNA gene Ammonia excretion Azotobacter Azotobacter - classification Azotobacter - genetics Azotobacter - isolation & purification BIOLOG Cotton-wheat cropping systems DNA Fingerprinting DNA, Bacterial - genetics DNA, Bacterial - isolation & purification DNA, Ribosomal - genetics DNA, Ribosomal - isolation & purification Genetic Variation Gossypium - genetics Gossypium - microbiology India Indole acetic acid Nitrogenase assay RNA, Ribosomal, 16S - genetics Soil Microbiology Triticum - genetics Triticum - microbiology |
| title | Diversity studies of Azotobacter spp. from cotton-wheat cropping systems of India |
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