Functional dissection of Hydrophilins during in vitro freeze protection

In plants, Late Embryogenesis Abundant (LEA) proteins typically accumulate in response to low water availability conditions imposed during development or by the environment. Analogous proteins in other organisms are induced when exposed to stress conditions. Most of this diverse set of proteins can...

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Published in:Plant, cell and environment cell and environment, 2008-12, Vol.31 (12), p.1781-1790
Main Authors: REYES, JOSÉ L, CAMPOS, FRANCISCO, WEI, HUI, ARORA, RAJEEV, YANG, YONGIL, KARLSON, DALE T, COVARRUBIAS, ALEJANDRA A
Format: Article
Language:English
Subjects:
Anilino Naphthalenesulfonates - metabolism
Arabidopsis - metabolism
Arabidopsis Proteins - metabolism
Biological and medical sciences
cryoprotectants
dehydrins
Freezing
freezing stress
Fundamental and applied biological sciences. Psychology
Hydrophilins
L-Lactate Dehydrogenase - metabolism
lactate dehydrogenase
LEA proteins
Plant Proteins - metabolism
Protein Structure, Secondary
Protein Structure, Tertiary
Rhododendron - metabolism
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Summary:In plants, Late Embryogenesis Abundant (LEA) proteins typically accumulate in response to low water availability conditions imposed during development or by the environment. Analogous proteins in other organisms are induced when exposed to stress conditions. Most of this diverse set of proteins can be grouped according to properties such as high hydrophilicity and high content of glycine or other small amino acids in what we have termed hydrophilins. Previously, we showed that hydrophilins protect enzyme activities in vitro from low water availability effects. Here, we demonstrate that hydrophilins can also protect enzyme activities from the adverse effects induced by freeze-thaw cycles in vitro. We monitored conformational changes induced by freeze-thaw on the enzyme lactate dehydrogenase (LDH) using the fluorophore 1-anilinonaphthalene-8-sulfonate (ANS). Hydrophilin addition prevents enzyme inactivation and this effect is reflected in changes in the ANS-fluorescence levels determined for LDH. We further show that for selected plant hydrophilins, removal of certain conserved domains affects their protecting capabilities. Thus, we propose that hydrophilins, and in particular specific protein domains, have a role in protecting cell components from the adverse effects caused by low water availability such as those present during freezing conditions by preventing deleterious changes in protein secondary and tertiary structure.
ISSN:0140-7791
1365-3040
DOI:10.1111/j.1365-3040.2008.01879.x