Engineering of Thermus thermophilus Cytochrome c₅₅₂: Thermally Tolerant Artificial Peroxidase

Customizing enzymes: We demonstrate that cytochrome c552 (Cyt c552) from Thermus thermophilus HB8 can be transformed into a thermally tolerant peroxidase by a design process modeled on the catalytic mechanism of peroxidases. At temperatures above 50 °C, the enzymatic activity of the engineered Cyt c...

Full description

Saved in:
Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2008-12, Vol.9 (18), p.2954-2957
Main Authors: Nakajima, Hiroshi, Ichikawa, Yusuke, Satake, Yuh, Takatani, Nobuyuki, Manna, Soumen Kanti, Rajbongshi, Jitumani, Mazumdar, Shyamalava, Watanabe, Yoshihito
Format: Article
Language:English
Subjects:
artificial metalloenzymes
Circular Dichroism
Cytochrome c Group - chemistry
Cytochrome c Group - genetics
cytochromes
Kinetics
Models, Molecular
peroxidases
Peroxidases - chemistry
Peroxidases - genetics
Protein Engineering
Temperature
Thermus thermophilus
Thermus thermophilus - enzymology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Customizing enzymes: We demonstrate that cytochrome c552 (Cyt c552) from Thermus thermophilus HB8 can be transformed into a thermally tolerant peroxidase by a design process modeled on the catalytic mechanism of peroxidases. At temperatures above 50 °C, the enzymatic activity of the engineered Cyt c552 surpasses that of a myoglobin variant that is known to exhibit the highest activity among artificial peroxidases.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.200800599