Rat endozepine-like peptide (ELP): cDNA cloning, genomic organization and tissue-specific expression

A cDNA encoding the rat homolog of the previously characterized murine endozepine-like peptide (ELP) was isolated by a PCR cloning strategy. Sequence comparison with the murine cDNA sequence revealed a conservation of the ELP primary structure between both rodent species with minor amino acid exchan...

Full description

Saved in:
Bibliographic Details
Published in:Gene 1999-07, Vol.235 (1), p.51-57
Main Authors: Pusch, W., Jähner, D., Spiess, A.-N., Ivell, R.
Format: Article
Language:English
Subjects:
5' Untranslated Regions - genetics
ACBP gene
Acyl-CoA binding protein
Amino Acid Sequence
Animals
Base Sequence
Carrier Proteins - chemistry
Carrier Proteins - genetics
Carrier Proteins - physiology
Cloning, Molecular
Diazepam Binding Inhibitor
DNA, Complementary - genetics
ELP gene
Endozepine
Endozepine-like peptide
Female
Gene Expression
Genome
Introns - genetics
Male
Mice
Molecular Sequence Data
Organ Specificity
Ovary
Ovary - metabolism
Proteins - chemistry
Proteins - genetics
Proteins - physiology
Rats
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sequence Homology, Amino Acid
Testis
Testis - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A cDNA encoding the rat homolog of the previously characterized murine endozepine-like peptide (ELP) was isolated by a PCR cloning strategy. Sequence comparison with the murine cDNA sequence revealed a conservation of the ELP primary structure between both rodent species with minor amino acid exchanges. We investigated the genomic organization of the rat ELP gene by genomic PCR. This indicated the presence of a single short intron of 451 bp interrupting the 5′ untranslated region. Tissue-dependent ELP expression was determined by Northern hybridization and semiquantitative RT-PCR. Northern hybridization showed an ELP specific transcript in both the male and the female gonad, but the level of ovarian ELP transcription was considerably lower than in the testis. RT-PCR analysis demonstrated a low and varying level of ELP background expression in all examined tissues. In contrast to the closely related ACBP gene, ELP shows a different genomic organization and a more regulated expression pattern, and may exert a specific function as a gonadal acyl-CoA pool former and transporter.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(99)00216-4