Thermococcus waiotapuensis sp. nov., an extremely thermophilic archaeon isolated from a freshwater hot spring

An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophi...

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Bibliographic Details
Published in:Archives of microbiology 1999-08, Vol.172 (2), p.95-101
Main Authors: GONZALEZ, J. M, SHECKELLS, D, VIEBAHN, M, KRUPATKINA, D, BORGES, K. M, ROBB, F. T
Format: Article
Language:English
Subjects:
Animal, plant and microbial ecology
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
DNA, Bacterial - genetics
DNA, Ribosomal - genetics
Freshwater
Fundamental and applied biological sciences. Psychology
Hot Temperature
Microbial ecology
Microbiology
Molecular Sequence Data
Phylogeny
RNA, Ribosomal, 16S - genetics
Space life sciences
Systematics
Thermococcus - classification
Thermococcus - genetics
Thermococcus - isolation & purification
Thermococcus - ultrastructure
Thermococcus waiotapuensis
Various environments (extraatmospheric space, air, water)
Water Microbiology
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Summary:An extremely thermophilic, sulfur-dependent archaeon, strain WT1, was isolated from a freshwater hot spring in the Lake Taupo area of North Island, New Zealand. The cells are flagellated, strictly anaerobic cocci that grow optimally at 85 degrees C and 5.4 g NaCl l(-1). The strain grows heterotrophically on complex proteinaceous substrates or on appropriate salts plus amino acid mixtures and is also able to utilize maltose, starch, and pyruvate. Elemental sulfur could be replaced by cystine or thioglycollate. The range of temperatures allowing growth is from 60 to 90 degrees C; the pH supporting growth ranges from 5 to 8 (optimum, pH 7). Strain WT1 grew in a defined medium containing amino acids as the sole carbon and energy sources. The required amino acids were: Arg, His, Ile, Leu, Phe, Ser, Thr, Trp, Tyr, and Val. Strain WT1 showed sensitivity to rifampicin. DNA G+C content was 50.4 mol%. Phylogenetic analysis of the sequence encoding the 16S rRNA gene indicated that this isolate is a member of the Thermococcales. DNA/DNA hybridization studies revealed no similarity to several species of Thermococcus and Pyrococcus, with the exception of Thermococcus zilligii. Based on the reported results, we propose strain WT1 as a new species to be named Thermococcus waiotapuensis sp. nov.
ISSN:0302-8933
1432-072X
DOI:10.1007/s002030050745