Quality control of inkjet technology for DNA microarray fabrication

• Published in: Biotechnology journal 2008-12, Vol.3 (12), p.1581-1590
• Main Authors: Pierik, Anke, Dijksman, Frits, Raaijmakers, Adrie, Wismans, Ton, Stapert, Henk
• Format: Article
• Language: English
• Subjects: Computer Peripherals; Equipment Design; Equipment Failure Analysis - methods; Equipment Failure Analysis - standards; Failure mode analysis; Inkjet printing; Microarrays; Microfluidics - instrumentation; Microfluidics - standards; Netherlands; Oligonucleotide Array Sequence Analysis - instrumentation; Oligonucleotide Array Sequence Analysis - standards; Oligonucleotides; Quality Control
• ISSN: 1860-6768; 1860-7314
• DOI: 10.1002/biot.200800121

A robust manufacturing process is essential to make high‐quality DNA microarrays, especially for use in diagnostic tests. We investigated different failure modes of the inkjet printing process used to manufacture low‐density microarrays. A single nozzle inkjet spotter was provided with two optical imaging systems, monitoring in real time the flight path of every droplet. If a droplet emission failure is detected, the printing process is automatically stopped. We analyzed over 1.3 million droplets. This information was used to investigate the performance of the inkjet system and to obtain detailed insight into the frequency and causes of jetting failures. Of all the substrates investigated, 96.2% were produced without any system or jetting failures. In 1.6% of the substrates, droplet emission failed and was correctly identified. Appropriate measures could then be taken to get the process back on track. In 2.2%, the imaging systems failed while droplet emission occurred correctly. In 0.1% of the substrates, droplet emission failure that was not timely detected occurred. Thus, the overall yield of the microarray manufacturing process was 99.9%, which is highly acceptable for prototyping.