The frequency, growth kinetics, and osteogenic/adipogenic differentiation properties of canine bone marrow stromal cells

Bone marrow stromal cells (BMSCs) have gained considerable attention as a potential source for cell transplantation therapies for a variety of diseases due to their accessibility, proliferative capacity, and multilineage differentiation properties. Canine BMSCs have been shown to contribute to regen...

Full description

Saved in:
Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2008-11, Vol.44 (10), p.472-479
Main Authors: Kamishina, Hiroaki, Farese, James P, Storm, Joshua A, Cheeseman, Jennifer A, Clemmons, Roger M
Format: Article
Language:English
Subjects:
Adipogenesis
Animal Genetics and Genomics
Animals
Biomedical and Life Sciences
Bone marrow
Bone Marrow Cells - cytology
Bones
Canine
Canines
Cell Biology
Cell Culture
Cell growth
Cell Growth/Differentiation/Apoptosis
Cell Proliferation
Cell Shape
Characterization
Colony-Forming Units Assay
Cultured cells
Developmental Biology
Differentiation
Dogs
Female
Hepatocytes
Kinetics
Life Sciences
Male
Mesenchymal stem cells
Osteogenesis
s
Stem Cells
Stromal cells
Stromal Cells - cytology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Bone marrow stromal cells (BMSCs) have gained considerable attention as a potential source for cell transplantation therapies for a variety of diseases due to their accessibility, proliferative capacity, and multilineage differentiation properties. Canine BMSCs have been shown to contribute to regeneration of osseous tissues, but knowledge about their biology is currently limited. In the present study, we investigated the frequency of adult canine BMSCs in bone marrow, morphological features, growth kinetics, and osteogenic as well as adipogenic differentiation properties in vitro. Our data suggest that adult canine bone marrow contains approximately one BMSC in every 2.38×104 bone marrow mononucleated cells (0.0042± 0.0019%, n=5). Primary BMSC cultures consisted of morphologically heterogeneous adherent cell populations from which spindle-shaped cells grew and became the predominant cell type. Growth kinetics patterns were dependent on the initial cell seeding densities, resulting in the highest fold increase at lower cell density. In the presence of osteogenic and adipogenic inducers, primary BMSCs underwent morphological and phenotypic changes characteristic of osteogenic and adipogenic differentiation, respectively. This study provides insights into basic characterization of adult canine BMSCs.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-008-9137-6