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Selective Release, Removal, and Reconstitution of Bacteriochlorophyll a Molecules into the B800 Sites of LH2 Complexes from Rhodopseudomonas acidophila 10050
A method is described which allows the selective release and removal of the Bchla-B800 molecules from the LH2 complex of Rhodopseudomonas acidophila 10050. This procedure also allows reconstitution of ∼80% of the empty binding sites with native Bchla. As shown by circular dichroism spectroscopy, the...
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| Published in: | Biochemistry (Easton) 1999-07, Vol.38 (30), p.9684-9692 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-a380t-7f3f8ad70f57fcd0ba1ed33f42e78d50cfa48913cdbbcaf289449b774bcb779d3 |
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| cites | cdi_FETCH-LOGICAL-a380t-7f3f8ad70f57fcd0ba1ed33f42e78d50cfa48913cdbbcaf289449b774bcb779d3 |
| container_end_page | 9692 |
| container_issue | 30 |
| container_start_page | 9684 |
| container_title | Biochemistry (Easton) |
| container_volume | 38 |
| creator | Fraser, Niall J Dominy, Peter J Ücker, Beaté Simonin, Ingrid Scheer, Hugo Cogdell, Richard J |
| description | A method is described which allows the selective release and removal of the Bchla-B800 molecules from the LH2 complex of Rhodopseudomonas acidophila 10050. This procedure also allows reconstitution of ∼80% of the empty binding sites with native Bchla. As shown by circular dichroism spectroscopy, the overall structures of the B850-only and reconstituted complexes are not affected by the pigment-exchange procedure. The pigments reconstituted into the B800 sites can also efficiently transfer excitation energy to the Bchla-B850 molecules. |
| doi_str_mv | 10.1021/bi990796i |
| format | article |
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The pigments reconstituted into the B800 sites can also efficiently transfer excitation energy to the Bchla-B850 molecules.</description><subject>Bacterial Proteins</subject><subject>bacteriochlorophyll a</subject><subject>Bacteriochlorophylls - chemistry</subject><subject>Bacteriochlorophylls - metabolism</subject><subject>Binding Sites</subject><subject>Buffers</subject><subject>Circular Dichroism</subject><subject>Detergents</subject><subject>Energy Transfer</subject><subject>Glucosides</subject><subject>Intracellular Membranes - chemistry</subject><subject>Intracellular Membranes - metabolism</subject><subject>Light-Harvesting Protein Complexes</subject><subject>Photosynthetic Reaction Center Complex Proteins - chemistry</subject><subject>Photosynthetic Reaction Center Complex Proteins - isolation & purification</subject><subject>Photosynthetic Reaction Center Complex Proteins - metabolism</subject><subject>Photosystem II Protein Complex</subject><subject>Rhodopseudomonas - chemistry</subject><subject>Rhodopseudomonas - metabolism</subject><subject>Rhodopseudomonas acidophila</subject><subject>Temperature</subject><subject>Time Factors</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNqFkc9uFSEUxonR2NvqwhcwbDQx6SgDzDAs7bVak2s099Y1YfiToTLDFJimfRjfVZppGhcmbuCcjx8f5HwAvKrR-xrh-kPvOEeMt-4J2NQNRhXlvHkKNgihtsK8RUfgOKWr0lLE6HNwVCOKCaZsA34fjDcquxsD96WSyZyWYgw30p9COenSqDCl7PKSXZhgsPBMqmyiC2rwIYZ5uPMeSvgtFJ_FmwTdlAPMg4FnHULw4HLRyrXdBYbbMM7e3BbBxjDC_RB0mJNZdBjDJBOUyhVhcF7CGqEGvQDPrPTJvHzYT8DPz-eX24tq9_3L1-3HXSVJh3LFLLGd1AzZhlmlUS9rowmxFBvW6QYpK2nHa6J03ytpcccp5T1jtFdl5ZqcgLer7xzD9WJSFqNLyngvJxOWJFrOcVPG91-wZqQjnDQFfLeCKoaUorFijm6U8U7USNyHJh5DK-zrB9OlH43-i1xTKkC1Ai5lc_t4LuMv0TLCGnH54yB2-NDuP2Eq7n_5ZuWlSuIqLHEqw_vHw38AcsmujA</recordid><startdate>19990727</startdate><enddate>19990727</enddate><creator>Fraser, Niall J</creator><creator>Dominy, Peter J</creator><creator>Ücker, Beaté</creator><creator>Simonin, Ingrid</creator><creator>Scheer, Hugo</creator><creator>Cogdell, Richard J</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19990727</creationdate><title>Selective Release, Removal, and Reconstitution of Bacteriochlorophyll a Molecules into the B800 Sites of LH2 Complexes from Rhodopseudomonas acidophila 10050</title><author>Fraser, Niall J ; Dominy, Peter J ; Ücker, Beaté ; Simonin, Ingrid ; Scheer, Hugo ; Cogdell, Richard J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a380t-7f3f8ad70f57fcd0ba1ed33f42e78d50cfa48913cdbbcaf289449b774bcb779d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Bacterial Proteins</topic><topic>bacteriochlorophyll a</topic><topic>Bacteriochlorophylls - chemistry</topic><topic>Bacteriochlorophylls - metabolism</topic><topic>Binding Sites</topic><topic>Buffers</topic><topic>Circular Dichroism</topic><topic>Detergents</topic><topic>Energy Transfer</topic><topic>Glucosides</topic><topic>Intracellular Membranes - chemistry</topic><topic>Intracellular Membranes - metabolism</topic><topic>Light-Harvesting Protein Complexes</topic><topic>Photosynthetic Reaction Center Complex Proteins - chemistry</topic><topic>Photosynthetic Reaction Center Complex Proteins - isolation & purification</topic><topic>Photosynthetic Reaction Center Complex Proteins - metabolism</topic><topic>Photosystem II Protein Complex</topic><topic>Rhodopseudomonas - chemistry</topic><topic>Rhodopseudomonas - metabolism</topic><topic>Rhodopseudomonas acidophila</topic><topic>Temperature</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fraser, Niall J</creatorcontrib><creatorcontrib>Dominy, Peter J</creatorcontrib><creatorcontrib>Ücker, Beaté</creatorcontrib><creatorcontrib>Simonin, Ingrid</creatorcontrib><creatorcontrib>Scheer, Hugo</creatorcontrib><creatorcontrib>Cogdell, Richard J</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemistry (Easton)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fraser, Niall J</au><au>Dominy, Peter J</au><au>Ücker, Beaté</au><au>Simonin, Ingrid</au><au>Scheer, Hugo</au><au>Cogdell, Richard J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selective Release, Removal, and Reconstitution of Bacteriochlorophyll a Molecules into the B800 Sites of LH2 Complexes from Rhodopseudomonas acidophila 10050</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1999-07-27</date><risdate>1999</risdate><volume>38</volume><issue>30</issue><spage>9684</spage><epage>9692</epage><pages>9684-9692</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>A method is described which allows the selective release and removal of the Bchla-B800 molecules from the LH2 complex of Rhodopseudomonas acidophila 10050. This procedure also allows reconstitution of ∼80% of the empty binding sites with native Bchla. As shown by circular dichroism spectroscopy, the overall structures of the B850-only and reconstituted complexes are not affected by the pigment-exchange procedure. The pigments reconstituted into the B800 sites can also efficiently transfer excitation energy to the Bchla-B850 molecules.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>10423247</pmid><doi>10.1021/bi990796i</doi><tpages>9</tpages></addata></record> |
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| ispartof | Biochemistry (Easton), 1999-07, Vol.38 (30), p.9684-9692 |
| issn | 0006-2960 1520-4995 |
| language | eng |
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| source | American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list) |
| subjects | Bacterial Proteins bacteriochlorophyll a Bacteriochlorophylls - chemistry Bacteriochlorophylls - metabolism Binding Sites Buffers Circular Dichroism Detergents Energy Transfer Glucosides Intracellular Membranes - chemistry Intracellular Membranes - metabolism Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins - chemistry Photosynthetic Reaction Center Complex Proteins - isolation & purification Photosynthetic Reaction Center Complex Proteins - metabolism Photosystem II Protein Complex Rhodopseudomonas - chemistry Rhodopseudomonas - metabolism Rhodopseudomonas acidophila Temperature Time Factors |
| title | Selective Release, Removal, and Reconstitution of Bacteriochlorophyll a Molecules into the B800 Sites of LH2 Complexes from Rhodopseudomonas acidophila 10050 |
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