Rab15 Mediates an Early Endocytic Event in Chinese Hamster Ovary Cells

Rab GTPases comprise a large family of monomeric proteins that regulate a diverse number of membrane trafficking events, including endocytosis. In this paper, we examine the subcellular distribution and function of the GTPase Rab15. Our biochemical and confocal immunofluorescence studies demonstrate...

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Bibliographic Details
Published in:The Journal of biological chemistry 1999-08, Vol.274 (32), p.22303-22312
Main Authors: Zuk, P A, Elferink, L A
Format: Article
Language:English
Subjects:
Animals
Biomarkers
Cell Compartmentation
Cell Fractionation
Centrioles - physiology
CHO Cells - physiology
Cricetinae
Endocytosis - physiology
Endosomes - physiology
Fluorescent Antibody Technique, Indirect
GTP Phosphohydrolases - genetics
GTP Phosphohydrolases - isolation & purification
GTP-Binding Proteins - genetics
GTP-Binding Proteins - isolation & purification
Mutation
rab GTP-Binding Proteins
rab4 GTP-Binding Proteins
rab5 GTP-Binding Proteins
Receptors, Transferrin - isolation & purification
Recombinant Proteins - isolation & purification
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Summary:Rab GTPases comprise a large family of monomeric proteins that regulate a diverse number of membrane trafficking events, including endocytosis. In this paper, we examine the subcellular distribution and function of the GTPase Rab15. Our biochemical and confocal immunofluorescence studies demonstrate that Rab15 associates with the transferrin receptor, a marker for the early endocytic pathway, but not with Rab7 or the cation-independent mannose 6-phosphate receptor, markers for late endosomal membranes. Furthermore, Rab15 colocalizes with Rab4 and -5 on early/sorting endosomes, as well as Rab11 on pericentriolar recycling endosomes. Consistent with its localization to early endosomal membranes, overexpression of the constitutively active mutant HArab15Q67L reduces receptor-mediated and fluid phase endocytosis. Therefore, our functional studies suggest that Rab15 may function as an inhibitory GTPase in early endocytic trafficking.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.274.32.22303