Scintigraphy and Immunohistology of Antimyosin-Fab During Graft Rejection

• Published in: Angiology 1999-07, Vol.50 (7), p.563-571
• Main Authors: Breuer, M., Schütz, A., Engelhardt, M., Hammer, C., Kemkes, B.M.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal; Biological and medical sciences; Biopsy; Disease Models, Animal; Dogs; Extracellular Space - immunology; Graft Rejection - diagnostic imaging; Graft Rejection - immunology; Graft Rejection - pathology; Heart Transplantation - diagnostic imaging; Heart Transplantation - immunology; Heart Transplantation - pathology; Immunoenzyme Techniques; Immunoglobulin Fab Fragments; Immunosuppressive Agents - therapeutic use; Indium Radioisotopes; Intracellular Fluid - immunology; Lung - diagnostic imaging; Medical sciences; Myosins - analysis; Neck; Radioimmunodetection; Radiopharmaceuticals; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the heart; T-Lymphocytes, Cytotoxic - immunology; T-Lymphocytes, Cytotoxic - pathology; Tomography, Emission-Computed, Single-Photon; Transplantation, Heterotopic - immunology; Transplantation, Heterotopic - pathology
• ISSN: 0003-3197; 1940-1574
• DOI: 10.1177/000331979905000706

Up to now, the cellular localization pattern of monoclonal antimyosin antibodies (AMA) during acute rejection has not been described. Focused on this the authors made immunohistochemical and scintigraphic studies (AMS) with AMA in an animal trans plantation model. Heterotopic cervical heart transplantation was performed in 12 mongrel dogs. Immunosuppression consisted of triple drug therapy. As standard the grafts were examined by daily transmural biopsies and routine histology. Dependent on the daily biopsy results, 0.5 mg of indium 111 (111In)-labeled AMA-Fab was injected. Subsequently every 2 hours transmural biopsy cylinders were taken out of the right ventricle and examined in indirect peroxidase staining technique. Forty-eight hours after AMA injection, scintigraphy in single photon emission computed tomography (SPECT) technique (AMS) was carried out and the heart-to-lung ratio (H/L-ratio) was calculated. The immunohistochemical maximum of AMA accumulation could be found 20 to 72 hours after AMA injection. This means that a scintigraphic examination should be done earlier than 20 hours and later than 3 days after injection. Dependent on the grades of bioptic rejection diagnosis a specific morphologic AMA localization was seen (grade I+II intercellular and slightly intracellular detection of AMA, grade III strongly intracellular and in particular perinuclear accumulation of the antibody, p