Regional Distribution in the Mouse of Proteins Homologous to Artery-Specific Antigenic Proteins (ASAPs)

We have reported the sequences of four novel proteins derived from extracts of human aortic tissue and a cDNA library from human aortic adventitia. These proteins are immunoreactive with serum immunoglobulins from patients with abdominal aortic aneurysms (AAA), and they have homologies of amino acid...

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Bibliographic Details
Published in:The Journal of surgical research 1999-08, Vol.85 (2), p.217-224
Main Authors: Borromeo, J.Roberto M., Koshy, Ninan, Park, Woosup M., Xia, Shichao, Hardy, Karen, Tilson, M.David
Format: Article
Language:English
Subjects:
Actin Cytoskeleton - metabolism
Animals
Antibodies - metabolism
Antibody Specificity
Aorta - metabolism
Binding, Competitive
Biological and medical sciences
Blood and lymphatic vessels
Cardiology. Vascular system
Cell Adhesion Molecules - immunology
Cell Adhesion Molecules - metabolism
Contractile Proteins - metabolism
Diseases of the aorta
Elastic Tissue - metabolism
Extracellular Matrix Proteins - immunology
Extracellular Matrix Proteins - metabolism
Humans
Immunoglobulins - immunology
Immunohistochemistry
Liver - metabolism
Medical sciences
Mice
Rabbits
Spleen - metabolism
Tissue Distribution
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Summary:We have reported the sequences of four novel proteins derived from extracts of human aortic tissue and a cDNA library from human aortic adventitia. These proteins are immunoreactive with serum immunoglobulins from patients with abdominal aortic aneurysms (AAA), and they have homologies of amino acid sequence with microfibrillar proteins of connective tissue. We are reporting separately that two of these proteins are artery-specific antigenic proteins (ASAPs) in man. The present work investigates the regional distribution of these two proteins (AAAP-40 and MatCAM-1) in mouse (E-beta-b). Antibodies were raised in rabbit against polypeptides encoding novel amino acid sequences, unique to these proteins (e.g., not reported in GenBank). Immunohistochemical studies with these two specific antibodies show conspicuous immunoreactivity of collagen-associated microfibrils in the aortic adventitia of the murine abdominal and thoracic aorta. Immunoreactive peptides were not present in brain, muscle, or kidney. These findings support the hypothesis that proteins occur in the mouse that are homologous to a unique family of aortic microfibrillar proteins in man.
ISSN:0022-4804
1095-8673
DOI:10.1006/jsre.1999.5659