IgY antiporcine endothelial cell antibodies effectively block human antiporcine xenoantibody binding

Avian IgY antibodies are structurally different from mammalian IgGs and do not fix mammalian complement components or bind human Fc receptors. As these antibody‐mediated interactions are believed to play significant roles in both hyperacute rejection (HAR) and acute vascular xenograft rejection (AVX...

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Bibliographic Details
Published in:Xenotransplantation (Københaven) 1999-05, Vol.6 (2), p.98-109
Main Authors: Fryer, Jonathan, Firca, Joseph, Leventhal, Joseph, Blondie, Beth, Malcolm, Andrew, Ivancic, David, Gandhi, Ripal, Shah, Ateet, Pao, Winnie, Abecassis, Michael, Kaufman, Dixon, Stuart, Frank, Anderson, Byron
Format: Article
Language:English
Subjects:
Animals
Antibodies, Heterophile - immunology
Antibodies, Heterophile - pharmacology
Antigens, Heterophile - immunology
Chickens
Complement Activation - drug effects
Complement Activation - immunology
Dose-Response Relationship, Drug
endothelial activation
endothelial cells
Endothelium, Vascular - immunology
Graft Rejection - immunology
Humans
IgY
Immunoglobulins - immunology
Immunoglobulins - pharmacology
Organ Transplantation
Rats
Transplantation Immunology
Transplantation, Heterologous
xenoantibodies
xenotransplantation
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Summary:Avian IgY antibodies are structurally different from mammalian IgGs and do not fix mammalian complement components or bind human Fc receptors. As these antibody‐mediated interactions are believed to play significant roles in both hyperacute rejection (HAR) and acute vascular xenograft rejection (AVXR), IgY antibodies to xenoantigen target epitopes may inhibit these rejection processes. In this report, we show that chicken IgY antibodies to α‐Gal antigen epitopes and to other porcine aortic endothelial cell (PAEC) antigens block human xenoreactive natural antibody binding to both porcine and rat cardiac tissues and porcine kidney tissues. Chicken IgY antibodies blocked complement‐mediated lysis of PAECs by human serum, and inhibited antibody‐dependent cell‐mediated lysis of PAECs by heat‐inactivated human serum plus peripheral blood leukocytes. Binding of IgY to porcine endothelial cells did not affect cell morphology nor expression of E‐selectin. These results suggest that avian IgYs could be of potential use in inhibiting pig‐to‐human xenograft rejection.
ISSN:0908-665X
1399-3089
DOI:10.1034/j.1399-3089.1999.00015.x