Localization of Entamoeba histolytica Amebopore in Amebic Liver Abscesses in Hamsters

• Published in: Annals of the New York Academy of Sciences 2008-12, Vol.1149 (1), p.375-379
• Main Authors: González, A., Monterrubio, D., Nequiz, M., López, R., Olivos, A., De Léon, C. García, Tello, E., Salaiza, N., Ramos, E., Gudiño, M., Montfort, I., Pérez-Tamayo, R.
• Format: Article
• Language: English
• Subjects: amebic liver abscesses; amebopore; Animals; Blotting, Western; Chromatography, Ion Exchange; Cricetinae; Electrophoresis, Polyacrylamide Gel; Entamoeba histolytica; Entamoeba histolytica - isolation & purification; Immunohistochemistry; Liver Abscess, Amebic - parasitology
• ISSN: 0077-8923; 1749-6632; 1930-6547
• DOI: 10.1196/annals.1428.006

Amebopore was purified from axenically grown trophozoites of the Entamoeba histolytica strain HM1:IMSS. The purification procedure involved Mono Q anion‐exchange chromatography and electroelution. Sequence analysis of the final product revealed that amebopore A was completely pure. Polyclonal antibodies against the purified amebopore were obtained from rabbits, and Western blot studies demonstrated their specificity. Sections of experimental, acute (1, 2, 3, and 4 days), amebic liver abscesses produced in hamsters were stained with the anti‐amebopore antibody; in all the analyzed stages, amebopore appeared as a constitutively expressed cytoplasmic molecule in trophozoites. No extracellular or hepatocyte‐membrane amebopore was found. This study is the first to trace amebopore in an in vivo model of amebic liver abscesses.