Comparative plasma membrane-associated proteomics of immortalized human hepatocytes

This work was initiated with the purpose of purifying and identifying differentially expressed plasma membrane-associated proteins between human liver cancer cell line HepG2 and normal liver cell line L02. The combined strategy of sucrose density gradient centrifugation and subsequent phase partitio...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2008-11, Vol.73 (11), p.1200-1206
Main Authors: Gou, Lan-Tu, Tong, Ai-Ping, Chen, Li-Juan, Tang, Ming-Hai, Chen, Bin, Liang, Shu-Fang, Huang, Canhua, Wei, Yu-Quan
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Cancer
Cell Line
Cell Line, Tumor
Cell Membrane - chemistry
Cell Membrane - metabolism
Centrifugation
Gene Expression Regulation
Hepatocytes - chemistry
Hepatocytes - cytology
Humans
Life Sciences
Liver cancer
Liver Neoplasms - chemistry
Liver Neoplasms - metabolism
Mass spectrometry
Membrane Proteins - analysis
Membrane Proteins - genetics
Membrane Proteins - isolation & purification
Membrane Proteins - metabolism
Membranes
Microbiology
Molecular Sequence Data
Plasma
Proteins
Proteome - analysis
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:This work was initiated with the purpose of purifying and identifying differentially expressed plasma membrane-associated proteins between human liver cancer cell line HepG2 and normal liver cell line L02. The combined strategy of sucrose density gradient centrifugation and subsequent phase partition was applied to obtain high-purity proteins of plasma membrane. Two-dimensional gel electrophoresis revealed the differential protein profile between the two cell lines. A total of 13 plasma membrane-associated proteins containing 10 up-regulated proteins and three down-regulated proteins in HepG2 cells were successfully identified by MALDI-Q-TOF mass spectrometry; they participate in multiple biological functions such as adhesion, proliferation, apoptosis, and signal transduction. The identified proteins could provide helpful reference in clinical investigations on potential candidates for diagnosis and therapy of liver cancer.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297908110059