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Human Regulatory CD8+ T Cells
Administration of a humanized monoclonal anti‐CD3 antibody (mAb) to patients with type 1 diabetes (T1D) increases their C‐peptide responses and the CD8/CD4 ratio. Incubation of human peripheral blood mononuclear cells (PBMC) with mAb in vitro has been shown to induce CD8+ regulatory T cells (Tregs)...
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Published in: | Annals of the New York Academy of Sciences 2008-12, Vol.1150 (1), p.234-238 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | Administration of a humanized monoclonal anti‐CD3 antibody (mAb) to patients with type 1 diabetes (T1D) increases their C‐peptide responses and the CD8/CD4 ratio. Incubation of human peripheral blood mononuclear cells (PBMC) with mAb in vitro has been shown to induce CD8+ regulatory T cells (Tregs) capable of inhibiting proliferation of CD4+ T cells. We hypothesized that CD8+ Tregs function through secretion of cytokines. To test that possibility, we generated CD8+ Tregs, sorted them by FACS, incubated them with syngeneic CD8‐depleted PBMC in the presence of staphylococcal enterotoxin B (SEB), and measured proliferation of T cells and cytokines. Using neutralizing anti‐cytokine mAbs, we show that the inhibitory effect of CD8+ Tregs could be partially alleviated by anti‐CCL‐4, anti‐TNF, and to a lesser extent anti‐IL2, suggesting that these cytokines contribute to CD8+ Treg function. |
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ISSN: | 0077-8923 1749-6632 1930-6547 |
DOI: | 10.1196/annals.1447.000 |