Application of high-performance liquid chromatography coupled to nuclear magnetic resonance spectrometry, mass spectrometry and bioassay for the determination of active saponins from Bacopa monniera Wettst

Reversed-phase high-performance liquid chromatographic separation coupled to (structurally informative) spectroscopic methods like NMR and MS and an efficient bioassay have been used to determine the active compounds from a crude fraction of Bacopa monniera. The fraction containing a mixture of sapo...

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Bibliographic Details
Published in:Journal of Chromatography A 1999-06, Vol.847 (1), p.109-116
Main Authors: Renukappa, T, Roos, G, Klaiber, I, Vogler, B, Kraus, W
Format: Article
Language:English
Subjects:
Animals
Anthelmintics - analysis
Anthelmintics - pharmacology
Bacopasaponins
Bacosides
Biological and medical sciences
Biological Assay - methods
Caenorhabditis elegans - drug effects
Carbohydrate Sequence
Chromatography, High Pressure Liquid - methods
General pharmacology
Magnetic Resonance Spectroscopy - methods
Mass Spectrometry - methods
Medical sciences
Molecular Sequence Data
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plants, Medicinal - chemistry
Saponins
Saponins - analysis
Saponins - pharmacology
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Summary:Reversed-phase high-performance liquid chromatographic separation coupled to (structurally informative) spectroscopic methods like NMR and MS and an efficient bioassay have been used to determine the active compounds from a crude fraction of Bacopa monniera. The fraction containing a mixture of saponins with closely related structures was found to show a significant anthelmintic activity against Caenorhabditis elegans (used as a model test organism for determining anthelmintic activity). The activity was correlated to two dammarane type triterpenoidal saponins containing at least three sugar units. The optimization of separation for 1 mg of the crude sample on column and the sensitivity of on-flow one- and two-dimensional NMR experiments to the high-molecular-mass compounds ( M r 890–930) has been demonstrated.
ISSN:0021-9673
DOI:10.1016/S0021-9673(99)00018-7