A simple visual immunoassay (VIA) for the semiquantitative determination of plasma elastase levels

Elastase levels are an important marker of the extent and severity of inflammatory processes. We describe a visual immunoassay based on two monoclonal antibodies for the semi-quantitative determination of plasma elastase levels. The method is rapid, reliable, easy to perform, and does not require sp...

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Bibliographic Details
Published in:Clinica chimica acta 1999-06, Vol.284 (1), p.93-100
Main Authors: Rojas, G, Flores, R M, Galván, J A, Vicedo, A, Gavilondo, J V
Format: Article
Language:English
Subjects:
Animals
Enzyme-Linked Immunosorbent Assay
Humans
Immunoassay - methods
Leukocyte Elastase - blood
Mice
Mice, Inbred BALB C
Point-of-Care Systems
Reproducibility of Results
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Summary:Elastase levels are an important marker of the extent and severity of inflammatory processes. We describe a visual immunoassay based on two monoclonal antibodies for the semi-quantitative determination of plasma elastase levels. The method is rapid, reliable, easy to perform, and does not require special laboratory equipment. There is coincidence of our results with those obtained by quantitative methods. The assay is suitable to obtain information for the management of patients in most intensive care units.
ISSN:0009-8981
DOI:10.1016/S0009-8981(99)00044-3