use of components of plant origin in the development of production technology for live cold-adapted cultural influenza vaccine

• Published in: Bulletin of experimental biology and medicine 2008-07, Vol.146 (1), p.144-147
• Main Authors: Mazurkova, N. A, Kolokol'tsova, T. D, Nechaeva, E. A, Shishkina, L. N, Sergeev, A. N
• Format: Article
• Language: English
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Cell Biology; Cell Line; Cold Temperature; Dogs; Female; Glycine max - chemistry; Humans; Influenza A virus; Influenza Vaccines; influenza virus; Influenza, Human - prevention & control; Internal Medicine; Laboratory Medicine; live cultural vaccine; MDCK cells; Orthomyxoviridae - physiology; Pathology; plant additives; Plant Proteins - chemistry; Plant Proteins - metabolism; Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine); Virus Replication
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-008-0223-2

The impact of culturing conditions (multiplicity of cell culture infection with influenza virus, composition of growth and maintenance nutrient media) for the efficiency of multiplication of cold-adapted reassortant vaccine strains of influenza A and B viruses was evaluated. Soybean hydrolysate protein-based biological additive to nutrient medium provided effective reproduction of influenza A virus in MDCK cells in the presence of 2 μg/ml trypsin. The use of soybean peptone-based stabilizer provided retention of infectious titer of influenza virus grown in MDCK culture after its lyophilization to a level of 8.5 lg EID₅₀/ml.