Cutting Edge: TCR Stimulation by Antibody and Bacterial Superantigen Induces Stat3 Activation in Human T Cells

Recent data show that TCR/CD3 stimulation induces activation of Stat5 in murine T cells. Here, we show that CD3 ligation by mAb and Staphylococcal enterotoxin (SE) induce a rapid, gradually accumulating, long-lasting tyrosine, and serine phosphorylation of Stat3 (but not Stat5) in allogen-specific h...

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Bibliographic Details
Published in:The Journal of immunology (1950) 1999-08, Vol.163 (4), p.1742-1745
Main Authors: Gerwien, Jens, Nielsen, Mette, Labuda, Tord, Nissen, Mogens H, Svejgaard, Arne, Geisler, Carsten, Ropke, Carsten, Odum, Niels
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - pharmacology
Binding Sites - genetics
Binding Sites - immunology
CD3 Complex - immunology
CD4-Positive T-Lymphocytes - immunology
CD4-Positive T-Lymphocytes - metabolism
Cell Line
DNA - metabolism
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Enterotoxins - pharmacology
Epitopes, T-Lymphocyte - metabolism
Humans
Interleukin-2 - pharmacology
Oligonucleotides - metabolism
Phosphorylation
Promoter Regions, Genetic - immunology
Receptors, Antigen, T-Cell - immunology
Receptors, Antigen, T-Cell - physiology
Signal Transduction - immunology
Staphylococcus aureus - immunology
STAT3 Transcription Factor
Superantigens - pharmacology
Trans-Activators - genetics
Trans-Activators - metabolism
Tyrosine - metabolism
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Summary:Recent data show that TCR/CD3 stimulation induces activation of Stat5 in murine T cells. Here, we show that CD3 ligation by mAb and Staphylococcal enterotoxin (SE) induce a rapid, gradually accumulating, long-lasting tyrosine, and serine phosphorylation of Stat3 (but not Stat5) in allogen-specific human CD4+ T cell lines. In contrast, IL-2 induces a rapid and transient tyrosine and serine phosphorylation of Stat3. Compared with IL-2, CD3 ligation induces a delayed Stat3 binding to oligonucleotide probes from the ICAM-1 and IL-2R alpha promoter. CD3-mediated activation of Stat3 is almost completely inhibited by a Src kinase inhibitor (PP1), whereas IL-2-induced Stat3 activation is unaffected. In conclusion, we show that CD3 ligation by mAb and SE triggers a rapid, PP1-sensitive tyrosine and serine phosphorylation of Stat3 in human CD4+ T cells. Moreover, we provide evidence that TCR/CD3 and IL-2 induce Stat3 activation via distinct signaling pathways.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.163.4.1742