Activities of key glycolytic enzymes in the brains of patients with Alzheimer's disease

The activities of hexokinase, aldolase, pyruvate kinase, lactate dehydrogenase and glucose 6-phosphate dehydrogenase were determined in brains of patients with Alzheimer's disease (AD) and in age matched controls. For pyruvate kinase and lactate dehydrogenase a significant increase in specific...

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Bibliographic Details
Published in:Journal of Neural Transmission 1999-01, Vol.106 (5-6), p.499-511
Main Authors: BIGL, M, BRÜCKNER, M. K, ARENDT, T, BIGL, V, ESCHRICH, K
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Alzheimer Disease - enzymology
Alzheimer Disease - pathology
Autopsy
Biological and medical sciences
Brain - enzymology
Brain - pathology
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Enzyme-Linked Immunosorbent Assay
Female
Frontal Lobe - enzymology
Fructose-Bisphosphate Aldolase - metabolism
Glucosephosphate Dehydrogenase - metabolism
Glycolysis
Hexokinase - metabolism
Hippocampus - enzymology
Humans
L-Lactate Dehydrogenase - metabolism
Male
Medical sciences
Neurology
Organ Specificity
Pyruvate Kinase - metabolism
Reference Values
Temporal Lobe - enzymology
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Summary:The activities of hexokinase, aldolase, pyruvate kinase, lactate dehydrogenase and glucose 6-phosphate dehydrogenase were determined in brains of patients with Alzheimer's disease (AD) and in age matched controls. For pyruvate kinase and lactate dehydrogenase a significant increase in specific activity was found in frontal and temporal cortex of AD brains, while the activities of aldolase and hexokinase are not changed. Glucose 6-phosphate dehydrogenase activity was significantly reduced in hippocampus. The increase of some glycolytic enzyme activities is correlated with increased contents of lactate dehydrogenase and glial fibrillary acidic protein (GFAP) in homogenates of frontal and temporal cortex and elevated phosphofructokinase (PFK) and GFAP in astrocytes from the same brain areas. The data extend previous findings on an increase in brain PFK specific activity in AD and suggest that the increased activity of some glycolytic enzymes may be, at least in part, the result of the reactive astrocytosis developing in the course of AD.
ISSN:0300-9564
1435-1463
DOI:10.1007/s007020050174