Cloning of murine CDK9/PITALRE and its tissue-specific expression in development

The cdc2‐family of serine/threonine kinases and their binding partners recently were implicated in developmental roles. We previously cloned a cdc2‐related kinase, cdk9/PITALRE, that is able to phosphorylate the retinoblastoma protein in vitro. We describe here the cloning and the characterization o...

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Bibliographic Details
Published in:Journal of cellular physiology 1998-11, Vol.177 (2), p.206-213
Main Authors: Bagella, Luigi, MacLachlan, Timothy K., Buono, Russell J., Pisano, M. Michele, Giordano, Antonio, De Luca, Antonio
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibody Specificity
Cell Differentiation - genetics
Cell Line
Cloning, Molecular
Cyclin-Dependent Kinase 9
DNA, Complementary - chemistry
DNA, Complementary - isolation & purification
Embryo, Mammalian - enzymology
Enzyme Activation
Female
Gene Expression Regulation, Developmental
Humans
Immunoblotting
Male
Mice
Mice, Inbred ICR
Molecular Sequence Data
Organ Specificity - genetics
Protein Kinases - biosynthesis
Protein Kinases - genetics
Protein Kinases - metabolism
Sequence Homology, Amino Acid
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Summary:The cdc2‐family of serine/threonine kinases and their binding partners recently were implicated in developmental roles. We previously cloned a cdc2‐related kinase, cdk9/PITALRE, that is able to phosphorylate the retinoblastoma protein in vitro. We describe here the cloning and the characterization of the mouse homolog of cdk9/PITALRE. The murine cDNA is 98% identical with humans and is expressed at high levels in brain and kidney tissues. The kinase activity and protein expression of cdk9/PITALRE were highest in terminally differentiated tissues such as the muscle and brain. In situ immunohistology and immunofluorescence detected cdk9/PITALRE protein not only within terminally differentiated cells such as muscle and neuronal cells, but also in proliferating cells. C2C12 and P19 cells induced to differentiate along muscle and neural lineages peaked in cdk9/PITALRE kinase activity at the end of differentiation. These results suggest that, among other roles, cdk9/PITALRE plays a role not unlike cdk5 in the differentiation of certain cell types. J. Cell. Physiol. 177:206–213, 1998. © 1998 Wiley‐Liss, Inc.
ISSN:0021-9541
1097-4652
DOI:10.1002/(SICI)1097-4652(199811)177:2<206::AID-JCP2>3.0.CO;2-R